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Literature summary for 3.2.1.23 extracted from

  • Schleper, C.; R๖der, R.; Singer, T.; Zillig, W.
    An insertion element of the extremely thermophilic archaeon Sulfolobus solfataricus transposes into the endogenous beta-galactosidase gene (1994), Mol. Gen. Genet., 243, 91-96.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lacS, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
additional information isolation of three phenotypically stable mutants by screening on activity against 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, isolation of the insertion sequence ISC1217 from the mutant enzymes, ISC1217 contains terminal inverted repeats and is flanked by a direct repeat of 6 bp, overview Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
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gene lacS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
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Saccharolobus solfataricus 5-bromo-4-chloro-indol + beta-D-galactose
-
?

Synonyms

Synonyms Comment Organism
beta-gal
-
Saccharolobus solfataricus