Literature summary for 3.2.1.23 extracted from

Todorova-Balvay, D.; Stoilova, I.; Gargova, S.; Vijayalakshmi, M.A.
An efficient two step purification and molecular characterization of beta-galactosidases from Aspergillus oryzae (2006), J. Mol. Recognit., 19, 299-304.

Search for more literature for 3.2.1.23

Application

Application	Comment	Organism
industry	beta-galactosidases are important industrial enzymes used for the hydrolysis of lactose from milk and milk whey, preparation of the improved mutant strain H26-10-7, overview	Aspergillus oryzae

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a mutant strain H26-10-7 from parental wild-type strains H26 and H-10-7, the enzyme from mutant strain H26-10-7 shows a 5fold increased activity and enhanced thermostability compared to the wild-type strain enzyme, overview	Aspergillus oryzae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Aspergillus oryzae	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
113000	-	gel filtration	Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
lactose + H2O	Aspergillus oryzae	-	D-glucose + D-galactose	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus oryzae	-	wild-type strains H26 and H-10-7, mutant strain H26-10-7	-

Purification (Commentary)

Purification (Comment)	Organism
enzyme from mutant strain H26-10-7 and from wild-type strain H26, 15.3fold and 12.8fold, respectively, by metal affinity chromatography and gel filtration	Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
40.2	-	purified enzyme from wild-type strain H26	Aspergillus oryzae
191.6	-	purified enzyme from mutant strain H26-10-7	Aspergillus oryzae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O	-	Aspergillus oryzae	2-nitrophenol + beta-D-galactose	-	?
lactose + H2O	-	Aspergillus oryzae	D-glucose + D-galactose	-	?
lactose + H2O	from milk and milk whey	Aspergillus oryzae	D-glucose + D-galactose	-	?

Synonyms

Synonyms	Comment	Organism
lactase	-	Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	10 min, 88% remaining activity of the mutant enzyme, 21% of the wild-type enzyme	Aspergillus oryzae
75	-	10 min, 32% remaining activity of the mutant enzyme, inactivation of the wild-type enzyme	Aspergillus oryzae
90	-	10 min, 10% remaining activity of the mutant enzyme, inactivation of the wild-type enzyme	Aspergillus oryzae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.6	4.8	assay at	Aspergillus oryzae

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Release 2023.1 (January 2023)