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Literature summary for 3.2.1.23 extracted from

  • Ladero, M.; Ruiz, G.; Pessela, B.C.; Vian, A.; Santos, A.; Garcia-Ochoa, F.
    Thermal and pH inactivation of an immobilized thermostable beta-galactosidase from Thermus sp. strain T2: Comparison to the free enzyme (2006), Biochem. Eng. J., 31, 14-24.
No PubMed abstract available
Search for more literature for 3.2.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain JM109 Thermus sp.

Protein Variants

Protein Variants Comment Organism
additional information enzyme immobilization on acid-cleaned, silanized support Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic modelling of the thermal and pH inactivation of the free and an immobilized form of the enzyme, detailed overview Thermus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose + H2O Thermus sp.
-
 D-glucose + D-galactose
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain JM109 Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermus sp. 2-nitrophenol + beta-D-galactose
-
 ?
lactose + H2O
-
 Thermus sp. D-glucose + D-galactose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Thermus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 90 kinetic modelling of the thermal and pH inactivation of the free and an immobilized form of the enzyme, inactivation mechanism, detailed overview Thermus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Thermus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5.5 kinetic modelling of the thermal and pH inactivation of the free and an immobilized form of the enzyme, inactivation mechanism, detailed overview Thermus sp.
9 13 kinetic modelling of the thermal and pH inactivation of the free and an immobilized form of the enzyme, inactivation mechanism, detailed overview Thermus sp.