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Literature summary for 3.2.1.23 extracted from

  • Chilaka, F.C.; Okeke, C.; Adaikpoh, E.
    Ligand-induced thermal stability in beta-galactosidase from the seeds of the black bean, Kerstingiella geocarpa (2002), Process Biochem., 38, 143-149.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
galactose competitive Kerstingiella geocarpa
glucose competitive at low concentrations, uncompetitive at high concentrations Kerstingiella geocarpa
lactose competitive Kerstingiella geocarpa

Organism

Organism UniProt Comment Textmining
Kerstingiella geocarpa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Kerstingiella geocarpa

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Kerstingiella geocarpa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.9
-
-
Kerstingiella geocarpa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl beta-D-galactopyranoside + H2O
-
Kerstingiella geocarpa p-nitrophenol + beta-D-galactose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Kerstingiella geocarpa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 40°C: about 45% of maximal activity, 65°C: about 45% of maximal activity Kerstingiella geocarpa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
galactose protects against irreversible thermoinactivation Kerstingiella geocarpa
60
-
2 h, no inactivation in presence of 100 mM galactose Kerstingiella geocarpa
80
-
1 h, complete inactivation. 47% of the activity regenerates within 6 h at 25°C Kerstingiella geocarpa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4
-
-
Kerstingiella geocarpa

pH Range

pH Minimum pH Maximum Comment Organism
3.8 5 pH 3.8: about 50% of maximal activity, pH 5.0: about 55% of maximal activity Kerstingiella geocarpa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
26
-
galactose
-
Kerstingiella geocarpa
49
-
glucose
-
Kerstingiella geocarpa
59.5
-
lactose
-
Kerstingiella geocarpa