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Literature summary for 3.2.1.23 extracted from
- Purification and characterization of a thermostable beta-galactosidase from kidney beans (Phaseolus vulgaris L.) cv. PDR14 (2003), J. Plant Physiol., 160, 327-337.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| galactose | competitive | Phaseolus vulgaris |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.63 | - |
p-nitrophenyl beta-D-galactopyranoside | pH 3.5, 37°C | Phaseolus vulgaris | |
| 0.74 | - |
o-nitrophenyl beta-D-galactopyranoside | pH 3.5, 37°C | Phaseolus vulgaris | |
| 50 | - |
lactose | - |
Phaseolus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
1 * 30000 + 1 * 45000, SDS-PAGE | Phaseolus vulgaris |
| 45000 | - |
1 * 30000 + 1 * 45000, SDS-PAGE | Phaseolus vulgaris |
| 75000 | - |
gel filtration | Phaseolus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
cv. PDR14 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Phaseolus vulgaris | D-glucose + D-galactose | - |
? | |
| o-nitrophenyl beta-D-galactopyranoside + H2O | - |
Phaseolus vulgaris | o-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl beta-D-galactopyranoside + H2O | - |
Phaseolus vulgaris | p-nitrophenol + beta-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 30000 + 1 * 45000, SDS-PAGE | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Phaseolus vulgaris |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
4°C, 24 h, stable | Phaseolus vulgaris |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.7 | - |
galactose | pH 3.5, 37°C | Phaseolus vulgaris | |
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Release 2023.1 (January 2023)
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