General Stability | Organism |
---|---|
10% inactivation after 4 h in 40% tetraglyme, 20% inactivation after 4 h in 70% tetraglyme, 50% inactivation after 4 h in 80% tetraglyme | Kluyveromyces marxianus |
15% inactivation after 4 h in 40% triethyl phosphate, 45% inactivation after 4 h in 50% triethyl phosphate, 52% inactivation after 4 h in 60% triethyl phosphate, 67% inactivation after 4 h in 70% triethyl phosphate, 85% inactivation after 4 h in 80% triethyl phosphate | Thermus sp. |
20% inactivation after 4 h in 40% triethyl phosphate, 42% inactivation after 4 h in 50% triethyl phosphate, 53% inactivation after 4 h in 60% triethyl phosphate, 64% inactivation after 4 h in 70% triethyl phosphate, 82% inactivation after 4 h in 80% triethyl phosphate | Kluyveromyces marxianus |
25% inactivation after 10 h in 0.8% Tween 80 in Tris-HCl buffer | Thermus sp. |
5% inactivation after 4 h in 60% tetraglyme, 35% inactivation after 4 h in 80% tetraglyme | Thermus sp. |
50% inactivation after 10 h in 5% SDS in Tris-HCl buffer | Thermus sp. |
87% inactivation after 10 h in 0.5% SDS in Tris-HCl buffer | Kluyveromyces marxianus |
unstable in 0.2 M urea | Kluyveromyces marxianus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.1 mM, 88% inhibition | Thermus sp. | |
Cu2+ | 0.1 mM, 55% inhibition | Thermus sp. | |
Mn2+ | 0.1 mM, 90% inhibition | Thermus sp. | |
SDS | 0.5 mM, 87% inactivation | Kluyveromyces marxianus | |
SDS | 1 mM, complete inactivation | Thermus sp. | |
Tween 80 | 0.5% in Tris-HCl, 25% inactivation | Thermus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 0.1 mM, activation to 118% of initial activity | Kluyveromyces marxianus | |
Co2+ | 0.1 mM, activation to 124% of initial activity | Kluyveromyces marxianus | |
Mn2+ | 0.1 mM, activation to 120% of initial activity | Kluyveromyces marxianus | |
Ni2+ | 0.1 mM, activation to 118% of initial activity | Kluyveromyces marxianus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
dimer, gel filtration | Thermus sp. |
200000 | - |
dimer, gel filtration | Kluyveromyces marxianus |
550000 | - |
octamer, gel filtration | Thermus sp. |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 5% inactivation after 4 h in 20% ethanol, 65% inactivation after 4 h in 40% ethanol, complete inactivation after 4 h in 60% ethanol | Kluyveromyces marxianus |
Ethanol | 5% inactivation after 4 h in 20% ethanol, 82% inactivation after 4 h in 60% ethanol | Thermus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces marxianus | - |
- |
- |
Thermus sp. | - |
- |
- |
Oxidation Stability | Organism |
---|---|
0.6% v/v H2O2 is very deleterious to the activity of the thermophilic enzyme | Thermus sp. |
0.6% v/v H2O2 sarcely affects the enzyme | Kluyveromyces marxianus |
Purification (Comment) | Organism |
---|---|
partial | Kluyveromyces marxianus |
partial | Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Kluyveromyces marxianus | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Thermus sp. | D-glucose + D-galactose | - |
? | |
additional information | enzyme shows a remarkably hydrolytic activity and a weak transgalactosylation activity, even in the presence of high concentrations of lactose, production of Gal-Gal and Gal-Gal-Glu | Kluyveromyces marxianus | ? | - |
? | |
additional information | enzyme shows a remarkably hydrolytic activity and a weak transgalactosylation activity, even in the presence of high concentrations of lactose, production of Gal-Gal-Glu | Thermus sp. | ? | - |
? | |
o-nitrophenyl beta-D-galactopyranoside + H2O | - |
Kluyveromyces marxianus | o-nitrophenol + beta-D-galactose | - |
? | |
o-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermus sp. | o-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl beta-D-galactopyranoside + H2O | - |
Kluyveromyces marxianus | p-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermus sp. | p-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Kluyveromyces marxianus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
about 30% loss of activity after 55 h | Kluyveromyces marxianus |
40 | - |
about 30% loss of activity after 5 h, about 75% loss of activity after 33 h | Kluyveromyces marxianus |
45 | - |
about 60% loss of activity after 5 h, about 90% loss of activity after 25 h | Kluyveromyces marxianus |
50 | - |
about 90% loss of activity after 2 hm complete inactivation agter 5 h | Kluyveromyces marxianus |
60 | - |
about 20% loss of activity after 20 h | Thermus sp. |
70 | - |
about 45% loss of activity after 20 h | Thermus sp. |
80 | - |
6 h, stable | Thermus sp. |
90 | - |
1 h, stable | Thermus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
hydrolysis of o-nitrophenyl beta-D-galactopyranoside | Thermus sp. |
6.5 | - |
hydrolysis of lactose | Thermus sp. |
6.5 | 6.8 | hydrolysis of lactose or o-nitrophenyl beta-D-galactopyranoside | Kluyveromyces marxianus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 2 | 70°C, about 30% loss of activity after 10 min, about 50% loss of activity after 1 h, about 75% loss of activity after 3 h | Thermus sp. |
4 | - |
70°C, about 20% loss of activity after 10 min, about 40% loss of activity after 1 h, about 75%loss of activity after 2 h, about 90% loss of activity after 3 h | Thermus sp. |
4 | - |
about 20% loss of activity after 10 min, about 85% loss of activity after 3 h | Kluyveromyces marxianus |
5 | - |
about 25% loss of activity after 1 h, about 60% of maximal activity after 3 h | Kluyveromyces marxianus |
6 | 10 | 70°C, 3 h, stable | Thermus sp. |
6 | - |
about 20% loss of activity after 3 h | Kluyveromyces marxianus |
7 | - |
about 5% loss of activity after 3 h | Kluyveromyces marxianus |
8 | - |
about 5% loss of activity after 3 h | Kluyveromyces marxianus |
9 | - |
about 35% loss of activity after 1 h, about 75% loss of activity after 3 h | Kluyveromyces marxianus |
10 | - |
about 65% loss of activity after 10 in, about 90% loss of activity after 1 h | Kluyveromyces marxianus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
Cu2+ | 70°C | Thermus sp. | |
1.85 | - |
Co2+ | 70°C | Thermus sp. | |
9.23 | - |
Mn2+ | 70°C | Thermus sp. |