Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.23 extracted from

  • Ladero, M.; Santos, A.; Garcia, J.L.; Carrascosa, A.V.; Pessela, B.C.C.; Garcia-Ochoa, F.
    Studies on the activity and the stability of beta-galactosidases from Thermus sp. strain T2 and from Kluyveromyces fragilis (2002), Enzyme Microb. Technol., 30, 392-405.
No PubMed abstract available

General Stability

General Stability Organism
10% inactivation after 4 h in 40% tetraglyme, 20% inactivation after 4 h in 70% tetraglyme, 50% inactivation after 4 h in 80% tetraglyme Kluyveromyces marxianus
15% inactivation after 4 h in 40% triethyl phosphate, 45% inactivation after 4 h in 50% triethyl phosphate, 52% inactivation after 4 h in 60% triethyl phosphate, 67% inactivation after 4 h in 70% triethyl phosphate, 85% inactivation after 4 h in 80% triethyl phosphate Thermus sp.
20% inactivation after 4 h in 40% triethyl phosphate, 42% inactivation after 4 h in 50% triethyl phosphate, 53% inactivation after 4 h in 60% triethyl phosphate, 64% inactivation after 4 h in 70% triethyl phosphate, 82% inactivation after 4 h in 80% triethyl phosphate Kluyveromyces marxianus
25% inactivation after 10 h in 0.8% Tween 80 in Tris-HCl buffer Thermus sp.
5% inactivation after 4 h in 60% tetraglyme, 35% inactivation after 4 h in 80% tetraglyme Thermus sp.
50% inactivation after 10 h in 5% SDS in Tris-HCl buffer Thermus sp.
87% inactivation after 10 h in 0.5% SDS in Tris-HCl buffer Kluyveromyces marxianus
unstable in 0.2 M urea Kluyveromyces marxianus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.1 mM, 88% inhibition Thermus sp.
Cu2+ 0.1 mM, 55% inhibition Thermus sp.
Mn2+ 0.1 mM, 90% inhibition Thermus sp.
SDS 0.5 mM, 87% inactivation Kluyveromyces marxianus
SDS 1 mM, complete inactivation Thermus sp.
Tween 80 0.5% in Tris-HCl, 25% inactivation Thermus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 0.1 mM, activation to 118% of initial activity Kluyveromyces marxianus
Co2+ 0.1 mM, activation to 124% of initial activity Kluyveromyces marxianus
Mn2+ 0.1 mM, activation to 120% of initial activity Kluyveromyces marxianus
Ni2+ 0.1 mM, activation to 118% of initial activity Kluyveromyces marxianus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
dimer, gel filtration Thermus sp.
200000
-
dimer, gel filtration Kluyveromyces marxianus
550000
-
octamer, gel filtration Thermus sp.

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol 5% inactivation after 4 h in 20% ethanol, 65% inactivation after 4 h in 40% ethanol, complete inactivation after 4 h in 60% ethanol Kluyveromyces marxianus
Ethanol 5% inactivation after 4 h in 20% ethanol, 82% inactivation after 4 h in 60% ethanol Thermus sp.

Organism

Organism UniProt Comment Textmining
Kluyveromyces marxianus
-
-
-
Thermus sp.
-
-
-

Oxidation Stability

Oxidation Stability Organism
0.6% v/v H2O2 is very deleterious to the activity of the thermophilic enzyme Thermus sp.
0.6% v/v H2O2 sarcely affects the enzyme Kluyveromyces marxianus

Purification (Commentary)

Purification (Comment) Organism
partial Kluyveromyces marxianus
partial Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
Kluyveromyces marxianus D-glucose + D-galactose
-
?
lactose + H2O
-
Thermus sp. D-glucose + D-galactose
-
?
additional information enzyme shows a remarkably hydrolytic activity and a weak transgalactosylation activity, even in the presence of high concentrations of lactose, production of Gal-Gal and Gal-Gal-Glu Kluyveromyces marxianus ?
-
?
additional information enzyme shows a remarkably hydrolytic activity and a weak transgalactosylation activity, even in the presence of high concentrations of lactose, production of Gal-Gal-Glu Thermus sp. ?
-
?
o-nitrophenyl beta-D-galactopyranoside + H2O
-
Kluyveromyces marxianus o-nitrophenol + beta-D-galactose
-
?
o-nitrophenyl beta-D-galactopyranoside + H2O
-
Thermus sp. o-nitrophenol + beta-D-galactose
-
?
p-nitrophenyl beta-D-galactopyranoside + H2O
-
Kluyveromyces marxianus p-nitrophenol + beta-D-galactose
-
?
p-nitrophenyl beta-D-galactopyranoside + H2O
-
Thermus sp. p-nitrophenol + beta-D-galactose
-
?

Subunits

Subunits Comment Organism
dimer
-
Kluyveromyces marxianus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
about 30% loss of activity after 55 h Kluyveromyces marxianus
40
-
about 30% loss of activity after 5 h, about 75% loss of activity after 33 h Kluyveromyces marxianus
45
-
about 60% loss of activity after 5 h, about 90% loss of activity after 25 h Kluyveromyces marxianus
50
-
about 90% loss of activity after 2 hm complete inactivation agter 5 h Kluyveromyces marxianus
60
-
about 20% loss of activity after 20 h Thermus sp.
70
-
about 45% loss of activity after 20 h Thermus sp.
80
-
6 h, stable Thermus sp.
90
-
1 h, stable Thermus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
hydrolysis of o-nitrophenyl beta-D-galactopyranoside Thermus sp.
6.5
-
hydrolysis of lactose Thermus sp.
6.5 6.8 hydrolysis of lactose or o-nitrophenyl beta-D-galactopyranoside Kluyveromyces marxianus

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 2 70°C, about 30% loss of activity after 10 min, about 50% loss of activity after 1 h, about 75% loss of activity after 3 h Thermus sp.
4
-
70°C, about 20% loss of activity after 10 min, about 40% loss of activity after 1 h, about 75%loss of activity after 2 h, about 90% loss of activity after 3 h Thermus sp.
4
-
about 20% loss of activity after 10 min, about 85% loss of activity after 3 h Kluyveromyces marxianus
5
-
about 25% loss of activity after 1 h, about 60% of maximal activity after 3 h Kluyveromyces marxianus
6 10 70°C, 3 h, stable Thermus sp.
6
-
about 20% loss of activity after 3 h Kluyveromyces marxianus
7
-
about 5% loss of activity after 3 h Kluyveromyces marxianus
8
-
about 5% loss of activity after 3 h Kluyveromyces marxianus
9
-
about 35% loss of activity after 1 h, about 75% loss of activity after 3 h Kluyveromyces marxianus
10
-
about 65% loss of activity after 10 in, about 90% loss of activity after 1 h Kluyveromyces marxianus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.14
-
Cu2+ 70°C Thermus sp.
1.85
-
Co2+ 70°C Thermus sp.
9.23
-
Mn2+ 70°C Thermus sp.