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Literature summary for 3.2.1.23 extracted from
- Purification and properties of a beta-D-galactosidase from Bifidobacterium bifidum exhibiting a transgalactosylation reaction (1994), Biotechnol. Appl. Biochem., 19, 341-354.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 5 mM, activates transferase activity | Bifidobacterium bifidum |  |
General Stability
| General Stability | Organism |
|---|---|
| lactose provides a heat-protective effect | Bifidobacterium bifidum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2.5 mM CaCl2, inhibition of transferase activity | Bifidobacterium bifidum | |
| Co2+ | - |
Bifidobacterium bifidum | |
| Mn2+ | 2.5 mM MnCl2, inhibition of transferase activity | Bifidobacterium bifidum | |
| Sn2+ | 2.5 mM SnCl2, inhibition of hydrolysis of p-nitrophenyl-beta-D-galactoside and of transferase activity | Bifidobacterium bifidum | |
| Zn2+ | 2.5 mM ZnCl2, inhibition of hydrolysis of p-nitrophenyl-beta-D-galactoside | Bifidobacterium bifidum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
p-nitrophenyl-beta-D-galactoside | - |
Bifidobacterium bifidum | |
| 13 | - |
lactose | hydrolysis | Bifidobacterium bifidum | |
| 800 | - |
lactose | transgalactosylation | Bifidobacterium bifidum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Bifidobacterium bifidum | 5622 | - |
| membrane | - |
Bifidobacterium bifidum | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 2.5 mM CaCl2, activates hydrolysis of lactose and p-nitrophenyl-beta-D-galactoside | Bifidobacterium bifidum | |
| Mn2+ | 2.5 mM MnCl2, activates hydrolysis of lactose and p-nitrophenyl-beta-D-galactoside | Bifidobacterium bifidum | |
| Sn2+ | 2.5 mM SnCl2, activates hydrolysis of lactose | Bifidobacterium bifidum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 362000 | - |
gel filtration | Bifidobacterium bifidum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium bifidum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bifidobacterium bifidum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + alpha-D-methyl-galactoside | transgalactosylation | Bifidobacterium bifidum | ? | - |
? | |
| lactose + alpha-D-methyl-glucoside | transgalactosylation | Bifidobacterium bifidum | ? | - |
? | |
| lactose + glucose | transgalactosylation | Bifidobacterium bifidum | ? | - |
? | |
| lactose + H2O | - |
Bifidobacterium bifidum | D-glucose + D-galactose | - |
? | |
| lactose + p-nitrophenyl beta-D-glucoside | transgalactosylation | Bifidobacterium bifidum | ? | - |
? | |
| lactose + sucrose | transgalactosylation | Bifidobacterium bifidum | ? | - |
? | |
| lactose + xylose | transgalactosylation | Bifidobacterium bifidum | ? | - |
? | |
| p-nitrophenyl-beta-D-galactoside + alpha-D-methyl-galactoside | transgalactosylation | Bifidobacterium bifidum | ? | - |
? | |
| p-nitrophenyl-beta-D-galactoside + alpha-D-methyl-glucoside | transgalactosylation | Bifidobacterium bifidum | ? | - |
? | |
| p-nitrophenyl-beta-D-galactoside + glucose | transgalactosylation | Bifidobacterium bifidum | ? | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Bifidobacterium bifidum | p-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + p-nitrophenyl beta-D-glucoside | transgalactosylation | Bifidobacterium bifidum | ? | - |
? | |
| p-nitrophenyl-beta-D-galactoside + sucrose | transgalactosylation | Bifidobacterium bifidum | ? | - |
? | |
| p-nitrophenyl-beta-D-galactoside + xylose | transgalactosylation | Bifidobacterium bifidum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 39 | hydrolysis of lactose or p-nitrophenyl-beta-D-galactoside | Bifidobacterium bifidum |
| 45 | - |
transgalactosylation | Bifidobacterium bifidum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
lactose provides a heat-protective effect | Bifidobacterium bifidum |
| 45 | - |
4 h, stable | Bifidobacterium bifidum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
transgalactosylation | Bifidobacterium bifidum |
| 6.5 | - |
hydrolysis of lactose or p-nitrophenyl-beta-D-galactoside | Bifidobacterium bifidum |
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