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Literature summary for 3.2.1.23 extracted from

  • Huber, R.E.; Roth, N.J.; Bahl, H.
    Quarternary structure, Mg2+ interactions, and some kinetic properties of the beta-galactosidase from Thermoanaerobacterium thermosulfurigenes EM1 (1996), J. Protein Chem., 15, 621-629.
    View publication on PubMed
Search for more literature for 3.2.1.23

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-D-galactose
-
 Thermoanaerobacterium thermosulfurigenes
D-arabinose
-
 Thermoanaerobacterium thermosulfurigenes
D-galactono-1,4-lactone
-
 Thermoanaerobacterium thermosulfurigenes
D-galactose
-
 Thermoanaerobacterium thermosulfurigenes
D-glucose
-
 Thermoanaerobacterium thermosulfurigenes
D-Lyxose
-
 Thermoanaerobacterium thermosulfurigenes
glycerol
-
 Thermoanaerobacterium thermosulfurigenes
isopropyl thiogalactoside
-
 Thermoanaerobacterium thermosulfurigenes
lactose
-
 Thermoanaerobacterium thermosulfurigenes
ribose
-
 Thermoanaerobacterium thermosulfurigenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 p-nitrophenyl-beta-D-galactoside at 50°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes
0.39
-
 p-nitrophenyl-beta-D-galactoside at 25°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes
0.58
-
 o-nitrophenyl-beta-D-galactoside at 50°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes
0.64
-
 o-nitrophenyl-beta-D-galactoside at 25°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes

Metals/Ions

Metals/Ions Comment Organism Structure
K+ no effect Thermoanaerobacterium thermosulfurigenes
Mg2+ contains 0.19 Mg2+ per monomer Thermoanaerobacterium thermosulfurigenes
Na+ no effect Thermoanaerobacterium thermosulfurigenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
175000 178000 gel filtration, non-denaturing PAGE Thermoanaerobacterium thermosulfurigenes

Organism

Organism UniProt Comment Textmining
Thermoanaerobacterium thermosulfurigenes
-
-
-
Thermoanaerobacterium thermosulfurigenes EM1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
o-nitrophenyl-beta-D-galactoside + H2O
-
 Thermoanaerobacterium thermosulfurigenes o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Thermoanaerobacterium thermosulfurigenes EM1 o-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Thermoanaerobacterium thermosulfurigenes p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Thermoanaerobacterium thermosulfurigenes EM1 p-nitrophenol + beta-D-galactose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
42.4
-
 p-nitrophenyl-beta-D-galactoside at 25°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes
54.5
-
 o-nitrophenyl-beta-D-galactoside at 25°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes
264
-
 o-nitrophenyl-beta-D-galactoside at 50°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes
312
-
 p-nitrophenyl-beta-D-galactoside at 50°C, in presence of 10 mM Mg2+ Thermoanaerobacterium thermosulfurigenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Thermoanaerobacterium thermosulfurigenes