Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Thermus sp. | |
Cys | - |
Thermus sp. | |
dithiothreitol | - |
Thermus sp. | |
EDTA | activates | Thermus sp. | |
phenylmethyl sulfonyl fluoride | - |
Thermus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | hydrolysis of p-nitrophenyl-beta-D-galactoside | Thermus sp. | |
Cu+ | - |
Thermus sp. | |
Cu2+ | - |
Thermus sp. | |
D-galactose | hydrolysis of p-nitrophenyl-beta-D-galactoside | Thermus sp. | |
D-glucose | hydrolysis of p-nitrophenyl-beta-D-galactoside | Thermus sp. | |
Fe2+ | - |
Thermus sp. | |
Fe3+ | weak | Thermus sp. | |
iodoacetate | - |
Thermus sp. | |
lactose | hydrolysis of p-nitrophenyl-beta-D-galactoside | Thermus sp. | |
Mn2+ | weak | Thermus sp. | |
Ni2+ | weak | Thermus sp. | |
PCMB | - |
Thermus sp. | |
xylose | hydrolysis of p-nitrophenyl-beta-D-galactoside | Thermus sp. | |
Zn2+ | - |
Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
o-nitrophenyl-beta-D-galactoside | soluble and immobilized enzyme | Thermus sp. | |
43 | - |
lactose | soluble and immobilized enzyme | Thermus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermus sp. | inducible enzyme | ? | - |
? | |
additional information | Thermus sp. 4-1A | inducible enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | - |
- |
- |
Thermus sp. 4-1A | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Thermus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62.83 | - |
- |
Thermus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | inducible enzyme | Thermus sp. | ? | - |
? | |
additional information | inducible enzyme | Thermus sp. 4-1A | ? | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermus sp. | o-nitrophenol + beta-D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermus sp. 4-1A | o-nitrophenol + beta-D-galactose | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
at pH 8, stable up to | Thermus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
soluble and immobilized enzyme | Thermus sp. |