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Literature summary for 3.2.1.23 extracted from

  • Cowan, D.A.; Daniel, R.M.; Martin, A.M.; Morgan, H.W.
    Some properties of a beta-galactosidase from an extremely thermophilic bacterium (1984), Biotechnol. Bioeng., 26, 1141-1145.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Thermus sp.
Cys
-
Thermus sp.
dithiothreitol
-
Thermus sp.
EDTA activates Thermus sp.
phenylmethyl sulfonyl fluoride
-
Thermus sp.

Inhibitors

Inhibitors Comment Organism Structure
cellobiose hydrolysis of p-nitrophenyl-beta-D-galactoside Thermus sp.
Cu+
-
Thermus sp.
Cu2+
-
Thermus sp.
D-galactose hydrolysis of p-nitrophenyl-beta-D-galactoside Thermus sp.
D-glucose hydrolysis of p-nitrophenyl-beta-D-galactoside Thermus sp.
Fe2+
-
Thermus sp.
Fe3+ weak Thermus sp.
iodoacetate
-
Thermus sp.
lactose hydrolysis of p-nitrophenyl-beta-D-galactoside Thermus sp.
Mn2+ weak Thermus sp.
Ni2+ weak Thermus sp.
PCMB
-
Thermus sp.
xylose hydrolysis of p-nitrophenyl-beta-D-galactoside Thermus sp.
Zn2+
-
Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5
-
o-nitrophenyl-beta-D-galactoside soluble and immobilized enzyme Thermus sp.
43
-
lactose soluble and immobilized enzyme Thermus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermus sp. inducible enzyme ?
-
?
additional information Thermus sp. 4-1A inducible enzyme ?
-
?

Organism

Organism UniProt Comment Textmining
Thermus sp.
-
-
-
Thermus sp. 4-1A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Thermus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62.83
-
-
Thermus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information inducible enzyme Thermus sp. ?
-
?
additional information inducible enzyme Thermus sp. 4-1A ?
-
?
o-nitrophenyl-beta-D-galactoside + H2O
-
Thermus sp. o-nitrophenol + beta-D-galactose
-
?
o-nitrophenyl-beta-D-galactoside + H2O
-
Thermus sp. 4-1A o-nitrophenol + beta-D-galactose
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
at pH 8, stable up to Thermus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
soluble and immobilized enzyme Thermus sp.