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Literature summary for 3.2.1.23 extracted from
- Physicochemical characterization of human intestinal lactase (1987), Biochem. J., 241, 567-572.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 170000 | - |
x * 170000, SDS-PAGE | Homo sapiens |
| 380000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestine | small intestine brush-border membrane | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 23 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | - |
Homo sapiens | 2 D-glucose | - |
? |  |
| lactose + H2O | - |
Homo sapiens | D-glucose + D-galactose | - |
? | |
| o-nitrophenyl beta-D-fucoside + H2O | - |
Homo sapiens | o-nitrophenol + beta-D-fucose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Homo sapiens | o-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Homo sapiens | p-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-glucoside + H2O | - |
Homo sapiens | p-nitrophenol + beta-D-glucose | - |
? | |
| phlorizin + H2O | - |
Homo sapiens | D-galactose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 170000, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
50 min, about 50% loss of phlorizin hydrolase activity, about 70% loss of lactase activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | about 75% of maximal activity at pH 5.0 and at pH 7.0 | Homo sapiens |
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Release 2023.1 (January 2023)
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