Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galactose | - |
Streptococcus thermophilus | |
Na+ | competitive inhibitor of the Mg2+ and K+ activated reaction with either lactose or p-nitrophenyl-beta-D-galactoside | Streptococcus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect of cations on the Km-value | Streptococcus thermophilus | |
7 | - |
lactose | in presence of 0.25 mM Mg2+, 50 mM K+ and 50 mM Na+ | Streptococcus thermophilus | |
13 | - |
lactose | in presence of 0.25 mM Mg2+ and 50 mM Na+ | Streptococcus thermophilus | |
19 | - |
lactose | in presence of 2 mM Mg2+ and 50 mM K+ | Streptococcus thermophilus | |
26 | - |
lactose | in presence of 0.25 mM Mg2+ and 50 mM K+ | Streptococcus thermophilus | |
67 | - |
lactose | in presence of 0.05 mM Mg2+ and 50 mM K+ | Streptococcus thermophilus | |
138 | - |
lactose | in presence of 0.25 mM Mg2+ | Streptococcus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | synergistic activation with Mg2+ and Na+ or Mg2+ and K+. Na+ is the better activator with either o-nitrophenyl-D-galactoside or 4-methylumbelliferyl-beta-D-galactoside as substrates while K+ is the better activator of lactose and p-nitrophenyl-beta-D-galactoside | Streptococcus thermophilus | |
Mg2+ | synergistic activation with Mg2+ and Na+ or Mg2+ and K+. Na+ is the better activator with either o-nitrophenyl-D-galactoside or 4-methylumbelliferyl-beta-D-galactoside as substrates while K+ is the better activator of lactose and p-nitrophenyl-beta-D-galactoside | Streptococcus thermophilus | |
Na+ | synergistic activation with Mg2+ and Na+ or Mg2+ and K+. Na+ is the better activator with either o-nitrophenyl-D-galactoside or 4-methylumbelliferyl-beta-D-galactoside as substrates while K+ is the better activator of lactose and p-nitrophenyl-beta-D-galactoside | Streptococcus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
116000 | - |
x * 116000, SDS-PAGE | Streptococcus thermophilus |
186000 | - |
enzyme form A2, gel filtration | Streptococcus thermophilus |
204000 | - |
enzyme form A1, gel filtration | Streptococcus thermophilus |
282000 | - |
enzyme form B1, gel filtration | Streptococcus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme form A1, A2, and B1 | Streptococcus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
466 | - |
- |
Streptococcus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-galactoside + H2O | - |
Streptococcus thermophilus | 4-methylumbelliferone + beta-D-galactose | - |
? | |
lactose + H2O | - |
Streptococcus thermophilus | D-glucose + D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Streptococcus thermophilus | o-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | - |
Streptococcus thermophilus | p-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 116000, SDS-PAGE | Streptococcus thermophilus |