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Literature summary for 3.2.1.23 extracted from
- Purification, crystallization, and properties of beta-galactosidase from Bacillus macerans (1988), Agric. Biol. Chem., 52, 625-631.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Paenibacillus macerans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 0.1 mM AgNO3, complete inhibition | Paenibacillus macerans |  |
| Cu2+ | 0.1 mM, complete inhibition | Paenibacillus macerans | |
| D-galactose | - |
Paenibacillus macerans | |
| Hg2+ | 0.1 mM HgCl2, complete inhibition | Paenibacillus macerans | |
| iodoacetate | - |
Paenibacillus macerans | |
| PCMB | - |
Paenibacillus macerans | |
| Tris | - |
Paenibacillus macerans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
p-nitrophenyl-beta-D-galactoside | - |
Paenibacillus macerans | |
| 30 | - |
lactose | - |
Paenibacillus macerans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
4 * 78000, SDS-PAGE | Paenibacillus macerans |
| 320000 | - |
gel filtration | Paenibacillus macerans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus macerans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paenibacillus macerans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 279.2 | - |
- |
Paenibacillus macerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Paenibacillus macerans | D-glucose + D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Paenibacillus macerans | p-nitrophenol + beta-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 78000, SDS-PAGE | Paenibacillus macerans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
in presence of 50 mM glycine, stable below | Paenibacillus macerans |
| 60 | - |
10 min, complete loss of activity | Paenibacillus macerans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Paenibacillus macerans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 30°C, 2 h, enzyme retains above 95% of its activity | Paenibacillus macerans |
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