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Literature summary for 3.2.1.23 extracted from

  • Kobayashi, T.; Hirose, Y.; Ohmiya, K.; Shimizu, S.; Uchino, F.
    Thermostable beta-galactosidase from Bacillus acidocaldarius and its immobilization (1978), J. Ferment. Technol., 56, 309-314.
No PubMed abstract available
Search for more literature for 3.2.1.23

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 lactose strain HM-1 Alicyclobacillus acidocaldarius
0.12
-
 lactose strain 11-1O Alicyclobacillus acidocaldarius

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius
-
 and strain 11-1O
-
Alicyclobacillus acidocaldarius HM-1
-
 and strain 11-1O
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
 Alicyclobacillus acidocaldarius D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Alicyclobacillus acidocaldarius HM-1 D-glucose + D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Alicyclobacillus acidocaldarius o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Alicyclobacillus acidocaldarius HM-1 o-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 strain HM-1 Alicyclobacillus acidocaldarius
70
-
 strain 11-1O Alicyclobacillus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life of immobilized enzyme from strain 11-1O at pH 6.5 when lactose solution is passed through the column is 42 days, when bovine milk is passed through the column the half-life is 32 days Alicyclobacillus acidocaldarius