Literature summary for 3.2.1.22 extracted from

Wang, H.; Luo, H.; Li, J.; Bai, Y.; Huang, H.; Shi, P.; Fan, Y.; Yao, B.
An alpha-galactosidase from an acidophilic Bispora sp. MEY-1 strain acts synergistically with beta-mannanase (2010), Biores. Technol., 101, 8376-8382.

Search for more literature for 3.2.1.22

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	a synergistic (3fold) increase in guar gum hydrolysis is observed when beta-mannanase Man5A from Bispora sp. MEY-1 and r-AgalB are combined	Bispora sp.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris	Bispora sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag2+	strongly inhibits AgalB activity	Bispora sp.
Fe3+	5 mM Fe3+ and Mn2+ decrease enzyme activity to 70% and 89% of its initial activity	Bispora sp.
Hg2+	strongly inhibits AgalB activity	Bispora sp.
Mn2+	5 mM Fe3+ and Mn2+ decrease enzyme activity to 70% and 89% of its initial activity	Bispora sp.
SDS	strongly inhibits AgalB activity	Bispora sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.1	-	4-nitrophenyl-alpha-D-galactopyranoside	pH 5.5, 37°C	Bispora sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	moderate stimulation	Bispora sp.
Ni2+	moderate stimulation	Bispora sp.
Zn2+	moderate stimulation	Bispora sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46100	-	deduced from cDNA	Bispora sp.
64000	-	SDS-PAGE	Bispora sp.

Organism

Organism	UniProt	Comment	Textmining
Bispora sp.	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Bispora sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl-alpha-D-galactopyranoside + H2O	-	Bispora sp.	4-nitrophenol + alpha-D-galactopyranose	-	?
guar gum + H2O	-	Bispora sp.	?	-	?
locust bean gum + H2O	-	Bispora sp.	?	-	?
melibiose + H2O	-	Bispora sp.	alpha-D-galactose + alpha-D-glucose	-	?
raffinose + H2O	-	Bispora sp.	sucrose + alpha-D-galactose	-	?
stachyose + 2 H2O	-	Bispora sp.	2 alpha-D-galactose + sucrose	-	?

Synonyms

Synonyms	Comment	Organism
AgalB	-	Bispora sp.
alpha-galactosidase	-	Bispora sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Bispora sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.5	-	-	Bispora sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
2.2	8	-	Bispora sp.

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Release 2023.1 (January 2023)