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Literature summary for 3.2.1.22 extracted from
- Characterization and biotechnological application of an acid alpha-galactosidase from Tachigali multijuga Benth. seeds (2008), Phytochemistry, 69, 2579-2585.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Tachigali multijuga |  |
| Cu2+ | - |
Tachigali multijuga | |
| D-galactose | competitive | Tachigali multijuga | |
| Hg2+ | - |
Tachigali multijuga | |
| melibiose | - |
Tachigali multijuga | |
| Sodium dodecyl sulfate | - |
Tachigali multijuga | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
4-nitrophenyl alpha-D-galactoside | pH 5.0, 40°C | Tachigali multijuga | |
| 5.37 | - |
melibiose | pH 5.0, 40°C | Tachigali multijuga | |
| 39.62 | - |
raffinose | pH 5.0, 40°C | Tachigali multijuga | |
| 48.8 | - |
stachyose | pH 5.0, 40°C | Tachigali multijuga | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Tachigali multijuga |
| 38000 | - |
1 * 38000 | Tachigali multijuga |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tachigali multijuga | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from germination seed | Tachigali multijuga |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | activity increases during seed germination and reaches a maximum level at 108 h after seed imbibition | Tachigali multijuga | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 83.6 | - |
pH 5.0, 40°C | Tachigali multijuga |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-galactoside + H2O | - |
Tachigali multijuga | 4-nitrophenol + alpha-D-galactose | - |
? | |
| galactomannan + H2O | - |
Tachigali multijuga | ? | - |
? | |
| melibiose + H2O | - |
Tachigali multijuga | D-galactose + D-glucose | - |
? | |
| raffinose + H2O | - |
Tachigali multijuga | sucrose + D-galactose | - |
? | |
| stachyose + H2O | - |
Tachigali multijuga | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38000 | Tachigali multijuga |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Tachigali multijuga |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable | Tachigali multijuga |
| 50 | - |
30 min, 79% loss of activity | Tachigali multijuga |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Tachigali multijuga |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
complete loss of activity | Tachigali multijuga |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.74 | - |
D-galactose | pH 5.0, 40°C | Tachigali multijuga | |
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