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Literature summary for 3.2.1.22 extracted from

  • Viana, P.A.; de Rezende, S.T.; Passos, F.M.; Oliveira, J.S.; Teixeira, K.N.; Santos, A.M.; Bemquerer, M.P.; Rosa, J.C.; Santoro, M.M.; Guimaraes, V.M.
    Debaryomyces hansenii UFV-1 intracellular alpha-galactosidase characterization and comparative studies with the extracellular enzyme (2009), J. Agric. Food Chem., 57, 2515-2522.
    View publication on PubMed
Search for more literature for 3.2.1.22

Application

Application Comment Organism
nutrition incubation of intracellular enzyme with soy milk for 6 h at 55°C reduces stachyose and raffinose amounts by 100% and 73%, respectively Debaryomyces hansenii

Inhibitors

Inhibitors Comment Organism Structure
Ag+ inactivation, intracellular enzyme Debaryomyces hansenii
Cu2+ inactivation, intracellular enzyme Debaryomyces hansenii
D-galactose competitive, intracellular enzyme Debaryomyces hansenii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 5.0, 55°C Debaryomyces hansenii
2.12
-
 melibiose pH 5.0, 55°C, intracellular enzyme Debaryomyces hansenii
10.8
-
 stachyose pH 5.0, 55°C, intracellular enzyme Debaryomyces hansenii
32.8
-
 raffinose pH 5.0, 55°C, intracellular enzyme Debaryomyces hansenii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Debaryomyces hansenii
-
-
intracellular
-
 Debaryomyces hansenii 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54500
-
 x * 54500, MALDI-TOF, x * 60000, SDS-PAGE, extracellular enzyme Debaryomyces hansenii
54800
-
 x * 54800, MALDI-TOF, x * 61000, SDS-PAGE, intracellular enzyme Debaryomyces hansenii
60000
-
 x * 54500, MALDI-TOF, x * 60000, SDS-PAGE, extracellular enzyme Debaryomyces hansenii
61000
-
 x * 54800, MALDI-TOF, x * 61000, SDS-PAGE, intracellular enzyme Debaryomyces hansenii

Organism

Organism UniProt Comment Textmining
Debaryomyces hansenii
-
-
-
Debaryomyces hansenii UFV-1
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein deglycosylated form presents 36000 Da molecular mass, corresponding to 40% carbohydrate content, extracellular enzyme Debaryomyces hansenii
glycoprotein deglycosylated form presents 40000 Da molecular mass, corresponding to 34% carbohydrate content, intracellular enzyme Debaryomyces hansenii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-D-galactopyranoside + H2O extracellular enzyme Debaryomyces hansenii 4-nitrophenol + D-galactopyranose
-
 ?
4-nitrophenyl-alpha-D-galactopyranoside + H2O intracellular enzyme Debaryomyces hansenii 4-nitrophenol + D-galactopyranose
-
 ?
4-nitrophenyl-alpha-D-galactopyranoside + H2O extracellular enzyme Debaryomyces hansenii UFV-1 4-nitrophenol + D-galactopyranose
-
 ?
4-nitrophenyl-alpha-D-galactopyranoside + H2O intracellular enzyme Debaryomyces hansenii UFV-1 4-nitrophenol + D-galactopyranose
-
 ?
melibiose + H2O intracellular enzyme Debaryomyces hansenii D-galactose + D-glucose
-
 ?
melibiose + H2O intracellular enzyme Debaryomyces hansenii UFV-1 D-galactose + D-glucose
-
 ?
raffinose + H2O intracellular enzyme Debaryomyces hansenii sucrose + D-galactose
-
 ?
raffinose + H2O intracellular enzyme Debaryomyces hansenii UFV-1 sucrose + D-galactose
-
 ?
stachyose + H2O intracellular enzyme Debaryomyces hansenii ?
-
 ?
stachyose + H2O intracellular enzyme Debaryomyces hansenii UFV-1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54500, MALDI-TOF, x * 60000, SDS-PAGE, extracellular enzyme Debaryomyces hansenii
? x * 54800, MALDI-TOF, x * 61000, SDS-PAGE, intracellular enzyme Debaryomyces hansenii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 intracellular enzyme Debaryomyces hansenii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 60 intracellular enzyme Debaryomyces hansenii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 melibiose pH 5.0, 55°C, intracellular enzyme Debaryomyces hansenii
3.29
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 5.0, 55°C, intracellular enzyme Debaryomyces hansenii
4.12
-
 raffinose pH 5.0, 55°C, intracellular enzyme Debaryomyces hansenii
6.05
-
 stachyose pH 5.0, 55°C, intracellular enzyme Debaryomyces hansenii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 intracellular enzyme Debaryomyces hansenii

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5.5 intracellular enzyme Debaryomyces hansenii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.7
-
 D-galactose pH 5.0, 55°C, intracellular enzyme Debaryomyces hansenii

pI Value

Organism Comment pI Value Maximum pI Value
Debaryomyces hansenii isoelectric focusing, intracellular enzyme
-
 4.1
Debaryomyces hansenii isoelectric focusing, extracellular enzyme
-
 5.1