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Literature summary for 3.2.1.22 extracted from

  • Cao, Y.; Wang, Y.; Meng, K.; Bai, Y.; Shi, P.; Luo, H.; Yang, P.; Zhou, Z.; Zhang, Z.; Yao, B.
    A novel protease-resistant alpha-galactosidase with high hydrolytic activity from Gibberella sp. F75: gene cloning, expression, and enzymatic characterization (2009), Appl. Microbiol. Biotechnol., 83, 875-884.
    View publication on PubMed
Search for more literature for 3.2.1.22

Activating Compound

Activating Compound Comment Organism Structure
Pb2+ 5 mM, significant enhancement of activity Fusarium sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Fusarium sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong Fusarium sp.
Fe3+ strong Fusarium sp.
Hg2+ strong Fusarium sp.
additional information enzyme is strongly resistant to alpha-chymotrypsin, subtilisin A, and collagenase digestion Fusarium sp.
Sodium dodecyl sulfate strong Fusarium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.06
-
 4-nitrophenyl-alpha-D-galactopyranoside 37°C Fusarium sp.
1.75
-
 melibiose 37°C Fusarium sp.
8.23
-
 stachyose 37°C Fusarium sp.
54.26
-
 raffinose 37°C Fusarium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82940
-
 or dimer, 3 * 82940, calculated Fusarium sp.
82940
-
 or trimer, 2 * 82940, calculated Fusarium sp.
220000
-
 and 320000, PAGE Fusarium sp.
320000
-
 and 220000, PAGE Fusarium sp.

Organism

Organism UniProt Comment Textmining
Fusarium sp. C6FJG8
-
-
Fusarium sp. F75 C6FJG8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48.33
-
 37°C Fusarium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
 Fusarium sp. 4-nitrophenol + D-galactopyranose
-
 ?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
 Fusarium sp. F75 4-nitrophenol + D-galactopyranose
-
 ?
6-bromo-2-naphthyl-alpha-D-galactopyranoside + H2O
-
 Fusarium sp. 6-bromo-2-naphthol + D-galactose
-
 ?
6-bromo-2-naphthyl-alpha-D-galactopyranoside + H2O
-
 Fusarium sp. F75 6-bromo-2-naphthol + D-galactose
-
 ?
melibiose + H2O
-
 Fusarium sp. D-galactose + D-glucose
-
 ?
melibiose + H2O
-
 Fusarium sp. F75 D-galactose + D-glucose
-
 ?
raffinose + H2O
-
 Fusarium sp. sucrose + D-galactose
-
 ?
raffinose + H2O
-
 Fusarium sp. F75 sucrose + D-galactose
-
 ?
stachyose + H2O
-
 Fusarium sp. ?
-
 ?
stachyose + H2O
-
 Fusarium sp. F75 ?
-
 ?

Subunits

Subunits Comment Organism
dimer or trimer, 2 * 82940, calculated Fusarium sp.
trimer or dimer, 3 * 82940, calculated Fusarium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Fusarium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37
-
 60% of maximum activity Fusarium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, no loss of activity Fusarium sp.
60
-
 30 min, 4.6% residual activity Fusarium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Fusarium sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 5 more than 80% of maximum activity Fusarium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 12
-
 Fusarium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Fusarium sp. calculated
-
 5.1