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Literature summary for 3.2.1.22 extracted from

  • Brouns, S.J.; Smits, N.; Wu, H.; Snijders, A.P; Wright, P.C.; de Vos, W.M.; van der Oost, J.
    Identification of a novel alpha-galactosidase from the hyperthermophilic archaeon Sulfolobus solfataricus. (2006), J. Bacteriol., 188, 2392-2399.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3)-RIL Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ complete inhibition at 10 mM Saccharolobus solfataricus
Ca2+ complete inhibition at 10 mM Saccharolobus solfataricus
Cd2+ complete inhibition at 10 mM Saccharolobus solfataricus
Co2+ complete inhibition at 10 mM Saccharolobus solfataricus
Cu2+ complete inhibition at 10 mM Saccharolobus solfataricus
Hg2+ complete inhibition at 10 mM Saccharolobus solfataricus
Mn2+ complete inhibition at 10 mM Saccharolobus solfataricus
additional information D-galactose, L-arabinose, D-fucose, sucrose, EDTA, dithiothreitol, Mg2+ and Mo2+ have no inhibitory effect Saccharolobus solfataricus
Ni2+ complete inhibition at 10 mM Saccharolobus solfataricus
Zn2+ complete inhibition at 10 mM Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.085
-
 p-nitrophenyl alpha-D-galactopyranoside in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C Saccharolobus solfataricus
37.4
-
 p-nitrophenyl-beta-L-arabinopyranoside in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
225000
-
 gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97U94
-
-
Saccharolobus solfataricus P2 Q97U94
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose Fast Flow column chromatography, hydroxyapatite column chromatography, Mono Q 5/50 GL column chromatography, and Superdex 200 HR 10/30 gel filtration Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.18
-
 crude cell extract, in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C Saccharolobus solfataricus
4.25
-
 after 23fold purification, in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
melibiose + H2O
-
 Saccharolobus solfataricus D-galactose + D-glucose
-
 ?
melibiose + H2O
-
 Saccharolobus solfataricus P2 D-galactose + D-glucose
-
 ?
additional information shows no activity toward p-nitrophenyl-alpha-substituted hexoses such as D-glucose, D-mannose, L-rhamnose, or N-acetyl-D-galactosaminide, p-nitrophenyl-beta-substituted hexoses such as D-galactose, D-glucose, and D-mannose do not support catalysis, nor does the pentose p-nitrophenyl-beta-D-xylopyranoside Saccharolobus solfataricus ?
-
 ?
additional information shows no activity toward p-nitrophenyl-alpha-substituted hexoses such as D-glucose, D-mannose, L-rhamnose, or N-acetyl-D-galactosaminide, p-nitrophenyl-beta-substituted hexoses such as D-galactose, D-glucose, and D-mannose do not support catalysis, nor does the pentose p-nitrophenyl-beta-D-xylopyranoside Saccharolobus solfataricus P2 ?
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus p-nitrophenol + alpha-D-galactose
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus P2 p-nitrophenol + alpha-D-galactose
-
 ?
p-nitrophenyl-beta-L-arabinopyranoside + H2O
-
 Saccharolobus solfataricus p-nitrophenol + beta-L-arabinose
-
 ?
raffinose + H2O
-
 Saccharolobus solfataricus sucrose + alpha-D-galactose
-
 ?
raffinose + H2O
-
 Saccharolobus solfataricus P2 sucrose + alpha-D-galactose
-
 ?
stachyose + 2 H2O
-
 Saccharolobus solfataricus 2 alpha-D-galactose + sucrose
-
 ?
stachyose + 2 H2O
-
 Saccharolobus solfataricus P2 2 alpha-D-galactose + sucrose
-
 ?

Subunits

Subunits Comment Organism
trimer
-
 Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
alpha-Gal
-
 Saccharolobus solfataricus
GalS
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 completely inactive at temperatures below 50°C Saccharolobus solfataricus
75
-
 50% active at 75°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.1 6.7 more than 50% active between pH 4.1 and 6.7 Saccharolobus solfataricus