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Literature summary for 3.2.1.22 extracted from
- Purification and properties of alpha-galactosidase from white-rot fungus Pleurotus florida (2007), Indian J. Biochem. Biophys., 44, 76-81.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-arabinose | the enzyme is strongly induced by L-arabinose | Pleurotus floridanus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | potential for the removal of raffinose and stachyose from soymilk | Pleurotus floridanus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | strong inhibitor | Pleurotus floridanus | |
| D-galactose | - |
Pleurotus floridanus | |
| D-glucose | - |
Pleurotus floridanus | |
| D-lactose | - |
Pleurotus floridanus | |
| D-maltose | - |
Pleurotus floridanus | |
| Hg2+ | strong inhibitor | Pleurotus floridanus | |
| N-bromosuccinimide | almost complete inhibition | Pleurotus floridanus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
p-nitrophenyl alpha-D-galactopyranoside | at 55°C | Pleurotus floridanus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 99000 | - |
SDS-PAGE | Pleurotus floridanus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pleurotus floridanus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acetone precipitation and DEAE-Sepharose column chromatography | Pleurotus floridanus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl alpha-D-galactopyranoside | - |
Pleurotus floridanus | p-nitrophenol + alpha-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 99000, SDS-PAGE | Pleurotus floridanus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Pleurotus floridanus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | loses 20% and 50% of the original activity in 30 min at 60°C and 70°C, respectively | Pleurotus floridanus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | 5 | - |
Pleurotus floridanus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | remains stable in a wide pH range (4.0-9.0) at 55°C for 2 h | Pleurotus floridanus |
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