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Literature summary for 3.2.1.22 extracted from

  • Carrera-Silva, E.A.; Silvestroni, A.; LeBlanc, J.G.; Piard, J.C.; Savoy de Giori, G.; Sesma, F.
    A thermostable alpha-galactosidase from Lactobacillus fermentum CRL722: genetic characterization and main properties (2006), Curr. Microbiol., 53, 374-378.
    View publication on PubMed
Search for more literature for 3.2.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Limosilactobacillus fermentum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 90.4% residual activity at 1 mM Limosilactobacillus fermentum
Cd2+ 86.0% residual activity at 1 mM Limosilactobacillus fermentum
Cu2+ 76.8% residual activity at 1 mM Limosilactobacillus fermentum
Hg+ 20.9% residual activity at 1 mM Limosilactobacillus fermentum
Li+ 93.3% residual activity at 1 mM Limosilactobacillus fermentum
Mg2+ 92.6% residual activity at 1 mM Limosilactobacillus fermentum
Mn2+ 91.5% residual activity at 1 mM Limosilactobacillus fermentum
Zn2+ 78.3% residual activity at 1 mM Limosilactobacillus fermentum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 4 * 84000, ultrafiltration Limosilactobacillus fermentum
336000
-
 native enzyme, ultrafiltration Limosilactobacillus fermentum

Organism

Organism UniProt Comment Textmining
Limosilactobacillus fermentum Q6IYF5
-
-
Limosilactobacillus fermentum CRL722 Q6IYF5
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose Fast Flow column chromatography and ammonium sulfate precipitation Limosilactobacillus fermentum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.21
-
 cell-free extract Limosilactobacillus fermentum
4.37
-
 after 20.8fold purification Limosilactobacillus fermentum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + phenyl alpha-D-galactoside
-
 Limosilactobacillus fermentum ?
-
 ?
D-galactose + phenyl alpha-D-galactoside
-
 Limosilactobacillus fermentum CRL722 ?
-
 ?
melibiose + H2O
-
 Limosilactobacillus fermentum D-galactose + D-glucose
-
 ?
melibiose + H2O
-
 Limosilactobacillus fermentum CRL722 D-galactose + D-glucose
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Limosilactobacillus fermentum p-nitrophenol + alpha-D-galactose
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Limosilactobacillus fermentum CRL722 p-nitrophenol + alpha-D-galactose
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 84000, ultrafiltration Limosilactobacillus fermentum

Synonyms

Synonyms Comment Organism
alpha-Gal
-
 Limosilactobacillus fermentum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Limosilactobacillus fermentum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 52 50% activity is observed between 35°C and 50°C Limosilactobacillus fermentum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 55 the enzyme is active at moderately high temperatures (55°C), no activity loss is observed after 30 min of incubation at 50°C, at 25°C the enzyme still keeps 20% activity, whereas at temperatures above 55°C it drastically decreases Limosilactobacillus fermentum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Limosilactobacillus fermentum

pH Range

pH Minimum pH Maximum Comment Organism
4.3 6.5 more than 50% activity between pH 4.3 and 6.5 Limosilactobacillus fermentum

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 20% activity at pH 8.0 Limosilactobacillus fermentum