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Literature summary for 3.2.1.22 extracted from

  • Chen, Y.; Jin, M.; Goodrich, L.; Smith, G.; Coppola, G.; Calhoun, D.H.
    Purification and characterization of human alpha-galactosidase A expressed in insect cells using a baculovirus vector (2000), Protein Expr. Purif., 20, 228-236.
    View publication on PubMed
Search for more literature for 3.2.1.22

Cloned(Commentary)

Cloned (Comment) Organism
cloned to an improved baculovirus vector and expressed in insect cells at optimized growth conditions, the purified enzyme is taken up by Fabry fibroblasts in culture resulting in normal enzyme levels Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46620
-
 laser desorption time-off-flight analysis Homo sapiens
46850
-
 electrospray mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens Fabry disease is a X-linked inborn error of glycolipid metabolism caused by deficiency of the lysosomal enzyme alpha-galactosidase A ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate accounts for about 10% of the mass of the glycoprotein. The carbohydrate moiety is composed of fucose, galactose, mannose, mannose-6-phosphate and N-acetylglucosamine. The human enzyme produced in insect cells has a higher fraction of complex structures, has a smaller fraction of high-mannose structures and contains a similar relative mass of carbohydrate compared to the enzyme produced in humans or vertebrate cell culture Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.052
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Fabry disease is a X-linked inborn error of glycolipid metabolism caused by deficiency of the lysosomal enzyme alpha-galactosidase A Homo sapiens ?
-
 ?