Any feedback?
Literature summary for 3.2.1.22 extracted from
- alpha-Galactosidases from the larval midgut of Tenebrio molitor (coleoptera) and Spodoptera frugiperda (lepidoptera) (2001), Comp. Biochem. Physiol. B, 128, 109-122.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-ethyl-3-(dimethylaminopropyl) carbodiimide | inactivation by 1-ethyl-3-(dimethylaminopropyl) carbodiimide plus glycine ethyl ester follows a two-phase kinetics: a fast one decreases in presence of melibiose and another slow not affected by melibiose | Spodoptera frugiperda |  |
| 1-ethyl-3-(dimethylaminopropyl) carbodiimide | inactivation by 1-ethyl-3-(dimethylaminopropyl) carbodiimide plus glycine ethyl ester follows a two-phase kinetics: a fast one decreases in presence of melibiose and another slow not affected by melibiose | Tenebrio molitor | |
| D-galactose | - |
Spodoptera frugiperda | |
| D-galactose | - |
Tenebrio molitor | |
| gamma-1,4-galactonolactone | - |
Spodoptera frugiperda | |
| gamma-1,4-galactonolactone | - |
Tenebrio molitor | |
| melibiose | with p-nitrophenyl alpha-D-galactopyranoside as substrate | Spodoptera frugiperda | |
| melibiose | with p-nitrophenyl alpha-D-galactopyranoside as substrate | Tenebrio molitor | |
| raffinose | with p-nitrophenyl alpha-D-galactopyranoside as substrate | Spodoptera frugiperda | |
| raffinose | with p-nitrophenyl alpha-D-galactopyranoside as substrate | Tenebrio molitor | |
| stachyose | with p-nitrophenyl alpha-D-galactopyranoside as substrate | Spodoptera frugiperda | |
| stachyose | with p-nitrophenyl alpha-D-galactopyranoside as substrate | Tenebrio molitor | |
| Tris | - |
Spodoptera frugiperda | |
| Tris | - |
Tenebrio molitor | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
melibiose | 30°C, pH 5.8 | Spodoptera frugiperda | |
| 3.2 | - |
p-nitrophenyl alpha-D-galactopyranoside | 30°C, pH 5.0 | Tenebrio molitor | |
| 3.8 | - |
stachyose | 30°C, pH 5.0 | Tenebrio molitor | |
| 4.4 | - |
raffinose | 30°C, pH 5.0 | Tenebrio molitor | |
| 4.8 | - |
raffinose | 30°C, pH 5.8 | Spodoptera frugiperda | |
| 6.3 | - |
melibiose | 30°C, pH 5.0 | Tenebrio molitor | |
| 7.2 | - |
stachyose | 30°C, pH 5.8 | Spodoptera frugiperda | |
| 12 | - |
p-nitrophenyl alpha-D-galactopyranoside | 30°C, pH 5.8 | Spodoptera frugiperda | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
2 * 46000 | Spodoptera frugiperda |
| 46000 | 49000 | gel filtration | Tenebrio molitor |
| 94000 | - |
ultracentrifugation | Spodoptera frugiperda |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spodoptera frugiperda | - |
- |
- |
| Tenebrio molitor | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | - |
Tenebrio molitor | - |
| larva | - |
Spodoptera frugiperda | - |
| midgut | of larva, three midgut alpha-galactosidases, SG1, SG2 and SG3 | Spodoptera frugiperda | - |
| midgut | of larva, three midgut alpha-galactosidases, TG1, TG2 and TG3 | Tenebrio molitor | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| melibiose + H2O | 7.3% of Vmax with p-nitrophenyl alpha-D-galactopyranoside | Tenebrio molitor | D-galactose + D-glucose | - |
? | |
| melibiose + H2O | 35.5% of Vmax with p-nitrophenyl alpha-D-galactopyranoside | Spodoptera frugiperda | alpha-D-galactose + D-glucose | - |
? | |
| p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Tenebrio molitor | p-nitrophenol + alpha-D-galactose | - |
? | |
| p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Spodoptera frugiperda | p-nitrophenol + alpha-D-galactose | - |
? | |
| raffinose + H2O | 20% of the Vmax with p-nitrophenyl alpha-D-galactopyranoside | Tenebrio molitor | sucrose + alpha-D-galactose | - |
? | |
| raffinose + H2O | Vmax is 1.8fold higher than that with p-nitrophenyl alpha-D-galactopyranoside | Spodoptera frugiperda | sucrose + alpha-D-galactose | - |
? | |
| stachyose + 2 H2O | 17.8% of the Vmax with p-nitrophenyl alpha-D-galactopyranoside | Tenebrio molitor | 2 alpha-D-galactose + sucrose | - |
? | |
| stachyose + 2 H2O | Vmax is 2fold higher than that with p-nitrophenyl alpha-D-galactopyranoside | Spodoptera frugiperda | 2 alpha-D-galactose + sucrose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 46000 | Spodoptera frugiperda |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Tenebrio molitor |
| 5.8 | - |
- |
Spodoptera frugiperda |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6.5 | pH 4.0: about 45% of maximal activity, pH 6.5: about 40% of maximal activity | Tenebrio molitor |
| 4.5 | 7.5 | pH 4.5: about 40% of maximal activity, pH 7.5: about 40% of maximal activity | Spodoptera frugiperda |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
30°C, 2 h, about 50% loss of activity | Tenebrio molitor |
| 4 | 6 | 30°C, 2 h, stable | Tenebrio molitor |
| 5 | 7 | stable | Spodoptera frugiperda |
| 7.5 | - |
30°C, 2 h, about 20% loss of activity | Tenebrio molitor |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
D-galactose | 30°C, pH 5.0 | Tenebrio molitor | |
| 5.6 | - |
D-galactose | 30°C, pH 5.8 | Spodoptera frugiperda | |
| 6.6 | - |
stachyose | 30°C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate | Spodoptera frugiperda | |
| 7.7 | - |
melibiose | 30°C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate | Tenebrio molitor | |
| 9 | - |
raffinose | 30°C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate | Spodoptera frugiperda | |
| 9.2 | - |
gamma-1,4-galactonolactone | 30°C, pH 5.0 | Tenebrio molitor | |
| 9.6 | - |
Tris | 30°C, pH 5.0 | Tenebrio molitor | |
| 10.3 | - |
melibiose | 30°C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate | Spodoptera frugiperda | |
| 11.6 | - |
stachyose | 30°C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate | Tenebrio molitor | |
| 12 | - |
Tris | 30°C, pH 5.8 | Spodoptera frugiperda | |
| 13.1 | - |
raffinose | 30°C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate | Tenebrio molitor | |
| 43.1 | - |
gamma-1,4-galactonolactone | 30°C, pH 5.8 | Spodoptera frugiperda | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Tenebrio molitor | isoelectric focusing | - |
4.6 |
| Tenebrio molitor | isoelectric focusing | - |
4.9 |
| Spodoptera frugiperda | isoelectric focusing | - |
7.1 |
| Spodoptera frugiperda | isoelectric focusing | - |
7.2 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
