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Literature summary for 3.2.1.22 extracted from

  • Kim, W.D.; Kaneko, S.; Park, G.G.; Tanaka, H.; Kusakabe, I.; Kobayashi, H.
    Purification and characterization of alpha-galactosidase from sunflower seeds (2003), Biotechnol. Lett., 25, 353-358.
    View publication on PubMed
Search for more literature for 3.2.1.22

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 1 * 40000, SDS-PAGE Helianthus annuus
41000
-
 gel filtration Helianthus annuus

Organism

Organism UniProt Comment Textmining
Helianthus annuus
-
 L. cv. Giant Gray Stripe
-

Purification (Commentary)

Purification (Comment) Organism
-
 Helianthus annuus

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Helianthus annuus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
85.8
-
-
 Helianthus annuus
104.9
-
 recombinant enzyme Helianthus annuus

Storage Stability

Storage Stability Organism
4°C, pH 5, less than 5% loss of activity after 6 months Helianthus annuus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]-beta-D-mannose + H2O
-
 Helianthus annuus alpha-D-galactopyranose + 4-O-[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]-beta-D-mannose
-
 ?
4-O-[4-O[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]beta-D-mannopyranosyl]-beta-D-mannose + H2O
-
 Helianthus annuus alpha-D-galactopyranose + 4-O-[4-O[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]beta-D-mannopyranosyl]-beta-D-mannose
-
 ?
melibiose + H2O
-
 Helianthus annuus alpha-D-galactose + D-glucose
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Helianthus annuus p-nitrophenol + alpha-D-galactose
-
 ?
raffinose + H2O
-
 Helianthus annuus sucrose + alpha-D-galactose
-
 ?
stachyose + 2 H2O
-
 Helianthus annuus 2 alpha-D-galactose + sucrose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Helianthus annuus

Synonyms

Synonyms Comment Organism
alpha-Gal
-
 Helianthus annuus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 1 h, stable up to Helianthus annuus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Helianthus annuus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 stable Helianthus annuus