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Literature summary for 3.2.1.22 extracted from

  • Smart, E.L.; Pharr, D.M.
    Characterization of alpha-Galactosidas from cucumber leaves (1980), Plant Physiol., 66, 731-734.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 p-nitrophenyl alpha-D-galactoside larger form Cucumis sativus
0.32
-
 p-nitrophenyl alpha-D-galactoside smaller form Cucumis sativus
3
-
 melibiose larger form Cucumis sativus
3.5
-
 melibiose smaller form Cucumis sativus
5
-
 raffinose smaller form Cucumis sativus
6.5
-
 raffinose larger form Cucumis sativus
10
-
 stachyose larger form Cucumis sativus
30
-
 stachyose smaller form Cucumis sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 smaller form, gel filtration Cucumis sativus
50000
-
 larger form, gel filtration Cucumis sativus

Organism

Organism UniProt Comment Textmining
Cucumis sativus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cucumis sativus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
230
-
 larger form Cucumis sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
melibiose + H2O
-
 Cucumis sativus D-galactose + D-glucose
-
 ?
p-nitrophenyl-alpha-D-galactoside + H2O
-
 Cucumis sativus p-nitrophenol + alpha-D-galactose
-
 ?
raffinose + H2O
-
 Cucumis sativus sucrose + D-galactose
-
 ?
stachyose + H2O
-
 Cucumis sativus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
 larger form Cucumis sativus
5.5
-
 smaller form Cucumis sativus