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Literature summary for 3.2.1.22 extracted from
- alpha-Galactosidase of Proterioochromonas malhamensis (1981), Phytochemistry, 20, 45-48.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-galactose | - |
Poterioochromonas malhamensis |  |
| EDTA | - |
Poterioochromonas malhamensis | |
| glycerol 3-phosphate | - |
Poterioochromonas malhamensis | |
| p-chloromercuribenzoate | - |
Poterioochromonas malhamensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
4-methylumbelliferyl alpha-D-galactoside | - |
Poterioochromonas malhamensis | |
| 0.71 | - |
p-nitrophenyl alpha-D-galactoside | - |
Poterioochromonas malhamensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Poterioochromonas malhamensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 360000 | - |
sucrose density-gradient centrifugation | Poterioochromonas malhamensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Poterioochromonas malhamensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Poterioochromonas malhamensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 30% glycerol or 10% sucrose, 3 months | Poterioochromonas malhamensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl alpha-D-galactoside + H2O | - |
Poterioochromonas malhamensis | 4-methylumbelliferone + D-galactose | - |
? | |
| p-nitrophenyl-alpha-D-galactoside + H2O | - |
Poterioochromonas malhamensis | p-nitrophenol + alpha-D-galactose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
1 h, pH 7, 55% loss in activity | Poterioochromonas malhamensis |
| 25 | - |
1 h, pH 7, 85% loss in activity | Poterioochromonas malhamensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Poterioochromonas malhamensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Poterioochromonas malhamensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Poterioochromonas malhamensis | |
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Release 2023.1 (January 2023)
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