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Literature summary for 3.2.1.22 extracted from
- Purification and properties of alpha-galactosidase from immature stalks of Saccharum officinarum (sugar cane) (1981), J. Biochem., 90, 1453-1461.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Saccharum officinarum |  |
| D-galactose | - |
Saccharum officinarum | |
| Hg2+ | - |
Saccharum officinarum | |
| L-ascorbic acid | - |
Saccharum officinarum | |
| melibiose | - |
Saccharum officinarum | |
| p-chloromercuribenzoate | - |
Saccharum officinarum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.83 | - |
p-nitrophenyl-alpha-D-galactopyranoside | - |
Saccharum officinarum | |
| 13 | - |
stachyose | - |
Saccharum officinarum | |
| 25.9 | - |
raffinose | - |
Saccharum officinarum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
gel filtration | Saccharum officinarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharum officinarum | - |
sugar cane | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharum officinarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30.6 | - |
- |
Saccharum officinarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| melibiose + H2O | - |
Saccharum officinarum | D-galactose + D-glucose | - |
? | |
| p-nitrophenyl-alpha-D-galactoside + H2O | - |
Saccharum officinarum | p-nitrophenol + alpha-D-galactose | - |
? | |
| raffinose + H2O | - |
Saccharum officinarum | sucrose + D-galactose | - |
? | |
| stachyose + H2O | - |
Saccharum officinarum | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Saccharum officinarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
quickly inactivated at higher temperatures | Saccharum officinarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Saccharum officinarum |
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