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Literature summary for 3.2.1.21 extracted from

  • Chan, A.; Wang, Y.; Ng, K.; Fu, Z.; Wong, W.
    Cloning and characterization of a novel cellobiase gene, cba3, encoding the first known beta-glucosidase of glycoside hydrolase family 1 of Cellulomonas biazotea (2012), Gene, 493, 52-61.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
gene cba3, DNA and amino acid sequence determination and analysis, genetic organization, functional expression of the extracellular His6-tagged enzyme in Escherichia coli strain JM101, transformants expressing recombinant Cba3 grow readily in minimal media using cellobiose as the sole carbon source Cellulomonas biazotea

Protein Variants

Protein Variants Comment Organism
additional information construction of inactive Cba3 deletants lacking either the N-terminal 10 amino acids or the C-terminal 10 residues Cellulomonas biazotea

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular despite lacking a classical N-terminal signal peptide, the enzyme is a secretory protein Cellulomonas biazotea
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + H2O Cellulomonas biazotea
-
 2 beta-D-glucose
-
 ?
cellobiose + H2O Cellulomonas biazotea ATCC 486
-
 2 beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Cellulomonas biazotea G1C1T5 gene cba3
-
Cellulomonas biazotea ATCC 486 G1C1T5 gene cba3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular His6-tagged enzyme from Escherichia coli strain JM101 culture supernatant by cobalt affinity chromatography, cleavage of the fusion protein with thrombin Cellulomonas biazotea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Cellulomonas biazotea 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Cellulomonas biazotea ATCC 486 4-nitrophenol + beta-D-glucose
-
 ?
cellobiose + H2O
-
 Cellulomonas biazotea 2 beta-D-glucose
-
 ?
cellobiose + H2O
-
 Cellulomonas biazotea ATCC 486 2 beta-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
Bgl
-
 Cellulomonas biazotea
Cba3
-
 Cellulomonas biazotea
cellobiase
-
 Cellulomonas biazotea
GH1 beta-glucosidase
-
 Cellulomonas biazotea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cellulomonas biazotea

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycoside hydrolase family 1, GH1 Cellulomonas biazotea
malfunction enzyme deletion mutants lacking either the N-terminal 10 amino acids or the C-terminal 10 residues are biologically inactive Cellulomonas biazotea