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Literature summary for 3.2.1.21 extracted from

  • Kim, H.W.; Ishikawa, K.
    Complete saccharification of cellulose at high temperature using endocellulase and beta-glucosidase from Pyrococcus sp. (2010), J. Microbiol. Biotechnol., 20, 889-892.
    View publication on PubMed
Search for more literature for 3.2.1.21

Application

Application Comment Organism
synthesis glucose production from cellulose material using beta-glucosidase from Pyrococcus furioses and endocellulase from Pyrococcus horikoshii. The combination reaction can produce only glucose without the other oligosaccharides from phosphoric acid swollen Avicel Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
20.3
-
 cellobiose pH 5.5, 85°C Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus E7FHY4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + H2O
-
 Pyrococcus furiosus 2 beta-D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
PF0073 locus name Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
 Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
594
-
 cellobiose pH 5.5, 85°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
29.3
-
 cellobiose pH 5.5, 85°C Pyrococcus furiosus