Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
daidzin | pH 5.5, 90°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56691 | - |
x * 56691, calculated from sequence | Saccharolobus solfataricus |
57000 | - |
x * 57000, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
179 | - |
pH 5.5, 90°C, substrate: daidzein | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
daidzein 7-O-(6-O-malonyl)beta-D-glucopyranoside + H2O | the specific activity of a recombinant enzyme for isoflavones in order of decreasing activity: daidzin, glycitin, genistin, malonyl genistin, malonyl daidzin, malonyl glycitin | Saccharolobus solfataricus | daidzein + 6-O-malonyl-beta-D-glucopyranose | - |
? | |
daidzin + H2O | the specific activity of a recombinant enzyme for isoflavones in order of decreasing activity: daidzin, glycitin, genistin, malonyl genistin, malonyl daidzin, malonyl glycitin | Saccharolobus solfataricus | beta-D-glucose + daidzein | - |
? | |
genistein 7-O-(6-O-malonyl)beta-D-glucopyranoside + H2O | the specific activity of a recombinant enzyme for isoflavones in order of decreasing activity: daidzin, glycitin, genistin, malonyl genistin, malonyl daidzin, malonyl glycitin | Saccharolobus solfataricus | beta-D-glucose + genistein | - |
? | |
genistin + H2O | the specific activity of a recombinant enzyme for isoflavones in order of decreasing activity: daidzin, glycitin, genistin, malonyl genistin, malonyl daidzin, malonyl glycitin | Saccharolobus solfataricus | beta-D-glucose + genistein | - |
? | |
glycitein 7-O-(6-O-malonyl)beta-D-glucopyranoside + H2O | the specific activity of a recombinant enzyme for isoflavones in order of decreasing activity: daidzin, glycitin, genistin, malonyl genistin, malonyl daidzin, malonyl glycitin | Saccharolobus solfataricus | beta-D-glucose + glycitein | - |
? | |
glycitin + H2O | the specific activity of a recombinant enzyme for isoflavones in order of decreasing activity: daidzin, glycitin, genistin, malonyl genistin, malonyl daidzin, malonyl glycitin | Saccharolobus solfataricus | beta-D-glucose + glycitein | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Saccharolobus solfataricus |
? | x * 56691, calculated from sequence | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life: 18 h | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2532 | - |
daidzin | pH 5.5, 90°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5065 | - |
daidzin | pH 5.5, 90°C | Saccharolobus solfataricus |