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Literature summary for 3.2.1.21 extracted from
- beta-Xylosidase activity of a GH3 glucosidase/xylosidase from yak rumen metagenome promotes the enzymatic degradation of hemicellulosic xylans (2012), Lett. Appl. Microbiol., 54, 79-87.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | the bifunctional beta-glucosidase/xylosidase can be used in simultaneous saccharification of cellulose and xylan into fermenantable glucose and xylose | uncultured rumen bacterium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.164 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 50°C, compared to KM-value: 0.03 mM for 4-nitrophenyl-beta-D-xylopyranoside of the beta-xylosidase activity, suggesting that RuBGX1 prefers 4-nitrophenyl-beta-D-xylopyranoside substrate | uncultured rumen bacterium |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
purified RuBGX1-6His | uncultured rumen bacterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-D-cellobioside + 2 H2O | uncultured rumen bacterium | native beta-glucosidase substrate, 14.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate | 4-methylumbelliferol + 2 D-glucose | - |
? | |
| cellobiose + H2O | uncultured rumen bacterium | native beta-glucosidase substrate, 26.4% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate | 2 D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured rumen bacterium | D3VYD8 | RuBGX1, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.21, as D272 is the catalytic nucleophile, and E167 and E481 are the catalytic acids; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | uncultured rumen bacterium | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-D-cellobioside + 2 H2O | native beta-glucosidase substrate, 14.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate | uncultured rumen bacterium | 4-methylumbelliferol + 2 D-glucose | - |
? | |
| 4-methylumbelliferyl beta-D-cellobioside + 2 H2O | native beta-glucosidase substrate, 14.9% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate | uncultured rumen bacterium | 4-methylumbelliferol + 2 D-glucose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | 8.3% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate | uncultured rumen bacterium | 4-nitrophenol + D-galactose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | 100% relative activity | uncultured rumen bacterium | 4-nitrophenol + D-glucose | - |
? | |
| cellobiose + H2O | native beta-glucosidase substrate, 26.4% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate | uncultured rumen bacterium | 2 D-glucose | - |
? | |
| additional information | no activity using 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, or sucrose as substrates | uncultured rumen bacterium | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-glucosidase | belongs to glycosyl hydrolase family 3, GH3 | uncultured rumen bacterium |
| bifunctional beta-glucosidase/xylosidase | - |
uncultured rumen bacterium |
| RuBGX1 | gene name, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.21 | uncultured rumen bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
a narrow optimum around 50°C | uncultured rumen bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 43 | 52 | 60% activity at 43°C, and 65% at 52°C, using p-nitrophenyl-beta-D-glucopyranoside as substrate | uncultured rumen bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
loses 38% activity after 10 min | uncultured rumen bacterium |
| 55 | - |
loses 90% after 3 min, indicating the RuBGX1 is a mesophilic enzyme | uncultured rumen bacterium |
| 60 | - |
loses 90% after 3 min, indicating the RuBGX1 is a mesophilic enzyme | uncultured rumen bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
4-nitrophenyl-beta-D-glucopyranoside as substrate | uncultured rumen bacterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | activity dropped to 22% at pH 8.0 and to near zero at pH 4.0 | uncultured rumen bacterium |
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