Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Oryza sativa |
Crystallization (Comment) | Organism |
---|---|
structures of the BGlu1 E176Q mutant, its complexes with cellotetraose, cellopentaose and laminaribiose, and its covalent intermediate with 2-deoxy-2-fluoroglucoside are determined at 1.65, 1.95, 1.80, 2.80, and 1.90 A resolution, respectively | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
E176A | inactive under standard assay conditions, mutant can be rescued by (small nucleophiles) acetate and azide but not as mutant E176Q | Oryza sativa |
E176D | inactive under standard assay conditions, mutant can not be rescued by any small nucleophile | Oryza sativa |
E176Q | E176Q active site mutant can be effectively rescued by small nucleophiles, such as acetate, azide and ascorbate, for hydrolysis of aryl glycosides in a pH-independent manner above pH 5. Cellotriose, cellotetraose, cellopentaose, cellohexaose and laminaribiose are not hydrolyzed by the mutant and instead exhibit competitive inhibition | Oryza sativa |
E386D | 3000fold less active than wild-type | Oryza sativa |
E386Q | 60000fold less active than wild-type | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Oryza sativa | 4-nitrophenol + D-glucopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase | - |
Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | in the presence of 50 mM azide, the apparent kcat values of the E176Q and E176A mutants are nearly independent of the pH from pH 5-10, while those of the wild-type and E176D enzymes decrease 30 and 8fold, respectively, as the pH increases from 5 to 10 | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Oryza sativa |