Literature summary for 3.2.1.21 extracted from

Choi, J.Y.; Park, A.R.; Kim, Y.J.; Kim, J.J.; Cha, C.J.; Yoon, J.J.
Purification and characterization of an extracellular beta-glucosidase produced by Phoma sp. KCTC11825BP isolated from rotten mandarin peel (2011), J. Microbiol. Biotechnol., 21, 503-508.

Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
-	Phoma sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
D-glucono-1,5-lactone	-	Phoma sp.
D-glucose	-	Phoma sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.3	-	4-nitrophenyl-beta-D-glucopyranoside	pH 4.5, 60°	Phoma sp.
3.2	-	cellobiose	pH 4.5, 60°	Phoma sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	inhibits activity	Phoma sp.
Mg2+	stimulates activity	Phoma sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
110000	-	subunit, SDS-PAGE	Phoma sp.
440000	-	native enzyme, tetramer, gel filtration	Phoma sp.

Organism

Organism	UniProt	Comment	Textmining
Phoma sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Phoma sp.

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Phoma sp.	4-nitrophenol + D-glucopyranose	-	?
cellobiose + H2O	-	Phoma sp.	2 beta-D-glucose	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 110000, gel filtration	Phoma sp.

Synonyms

Synonyms	Comment	Organism
beta-glucosidase	-	Phoma sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Phoma sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	enzyme has a half-life of 53 h at 60°C	Phoma sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.5	-	4-nitrophenyl-beta-D-glucopyranoside	pH 4.5, 60°	Phoma sp.
699	-	cellobiose	pH 4.5, 60°	Phoma sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	-	Phoma sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.1	-	D-glucono-1,5-lactone	pH 4.5, 60°	Phoma sp.
1.7	-	D-glucose	pH 4.5, 60°	Phoma sp.

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Release 2023.1 (January 2023)