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Literature summary for 3.2.1.21 extracted from
- Cloning and characterization of a beta-glucosidase from marine microbial metagenome with excellent glucose tolerance (2010), J. Microbiol. Biotechnol., 20, 1351-1358.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | at concentrations lower than 400 mM | uncultured bacterium |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | uncultured bacterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | at concentrations higher than 400 mM | uncultured bacterium | |
| ethanol | - |
uncultured bacterium | |
| SDS | - |
uncultured bacterium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.39 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.5, 40°C | uncultured bacterium | |
| 20.4 | - |
cellobiose | pH 6.5, 40°C | uncultured bacterium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates activity | uncultured bacterium | |
| Cu2+ | inhibits activity | uncultured bacterium | |
| K+ | stimulates activity | uncultured bacterium | |
| Mg2+ | stimulates activity | uncultured bacterium | |
| NaCl | loss 86% of its activity in the presence of 500 mM NaCl | uncultured bacterium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49900 | - |
calculated from cDNA | uncultured bacterium |
| 51000 | - |
SDS-PAGE | uncultured bacterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | D5KX75 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
uncultured bacterium | 4-nitrophenol + D-glucopyranose | - |
? | |
| cellobiose + H2O | - |
uncultured bacterium | 2 beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | native-PAGE shows Bgl1A in the form of a monomer and dimer | uncultured bacterium |
| monomer | native-PAGE shows Bgl1A in the form of a monomer and dimer | uncultured bacterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-glucosidase | - |
uncultured bacterium |
| BGL1A | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
optimal temperature | uncultured bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 55 | - |
uncultured bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
the enzyme is stable at temperatures lower than 15°C, as it retains 70% of the original activity after incubation at pH 7.5 and 15°C for 1 h | uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
optimal pH | uncultured bacterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | - |
uncultured bacterium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
uncultured bacterium |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1000 | - |
D-glucose | pH 6.5, 40°C | uncultured bacterium | |
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