Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | inhibits activity | Olea europaea | |
Cd2+ | inhibits activity | Olea europaea | |
Cu2+ | inhibits activity | Olea europaea | |
D-glucono-1,5-lactone | - |
Olea europaea | |
D-glucose | - |
Olea europaea | |
Ni2+ | inhibits activity | Olea europaea | |
Pb2+ | inhibits activity | Olea europaea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.22 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 37°C | Olea europaea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | increases activity | Olea europaea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
SDS-PAGE | Olea europaea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Olea europaea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified by salting out with ammonium sulfate and using specifically designed Sepharose-4B-l-tyrosine-1-naphthylamine hydrophobic interaction chromatography. Purification: 155fold, enzyme yield: 54% | Olea europaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Olea europaea | 2-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Olea europaea | 4-nitrophenol + D-glucopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase | - |
Olea europaea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000104 | - |
Ni2+ | pH 5.5, 37°C | Olea europaea | |
0.000226 | - |
Cu2+ | pH 5.5, 37°C | Olea europaea | |
0.000429 | - |
Cd2+ | pH 5.5, 37°C | Olea europaea | |
0.00321 | - |
Ag+ | pH 5.5, 37°C | Olea europaea | |
0.0126 | - |
Pb2+ | pH 5.5, 37°C | Olea europaea | |
0.016 | - |
D-glucono-1,5-lactone | pH 5.5, 37°C | Olea europaea | |
6.4 | - |
D-glucose | pH 5.5, 37°C | Olea europaea |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000038 | - |
pH 5.5, 37°C | Olea europaea | Cd2+ | |
0.000063 | - |
pH 5.5, 37°C | Olea europaea | Ni2+ | |
0.00061 | - |
pH 5.5, 37°C | Olea europaea | Cu2+ | |
0.00334 | - |
pH 5.5, 37°C | Olea europaea | Ag+ | |
0.0053 | - |
pH 5.5, 37°C | Olea europaea | Pb2+ | |
0.23 | - |
pH 5.5, 37°C | Olea europaea | D-glucono-1,5-lactone | |
105.5 | - |
pH 5.5, 37°C | Olea europaea | D-glucose |