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Literature summary for 3.2.1.21 extracted from

  • Seshadri, S.; Akiyama, T.; Opassiri, R.; Kuaprasert, B.; Cairns, J.K.
    Structural and enzymatic characterization of Os3BGlu6, a rice beta-glucosidase hydrolyzing hydrophobic glycosides and (1->3)- and (1->2)-linked disaccharides (2009), Plant Physiol., 151, 47-58.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Origami (DE3) cells Oryza sativa

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with a covalent intermediate, 2-deoxy-2-fluoroglucoside and, n-octyl-beta-D-thioglucopyranoside, microbatch under oil method, using 0.1 M Bis-Tris, pH 6.5, and 20% PEG MME 5000 Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 4-nitrophenyl-beta-D-fucopyranoside in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
3.6
-
 laminaribiose beta-(1-3)-linked laminaribose,in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
5
-
 n-heptyl-beta-D-glucoside in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
6.06
-
 4-nitrophenyl-beta-D-galactopyranoside in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
6.3
-
 4-nitrophenyl-beta-D-glucopyranoside in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
8.7
-
 laminaritriose in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
9.8
-
 sophorose beta-(1-2)-linked sophorose, in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
14.9
-
 gentiobiose beta-(1-6)-linked gentiobiose,in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
15.3
-
 cellobiose beta-(1-4)-linked cellobiose,in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 SDS-PAGE Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q8L7J2 cultivar Yukihikari
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal (Co2+) affinity chromatography on Talon resin Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-fucopyranoside + H2O 415% activity compared to 4-nitrophenyl beta-D-glucopyranoside Oryza sativa 4-nitrophenol + beta-D-fucopyranose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O 29.4% activity compared to 4-nitrophenyl beta-D-glucopyranoside Oryza sativa 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O 100% activity Oryza sativa 4-nitrophenol + D-glucopyranose
-
 ?
cellobiose [beta-(1-4)-linked] + H2O beta-(1-4)-linked cellobiose Oryza sativa ?
-
 ?
gentiobiose + H2O beta-(1-6)-linked gentiobiose Oryza sativa ?
-
 ?
laminaribiose + H2O beta-(1-3)-linked laminaribose, 55.7% activity compared to n-octyl beta-D-glucopyranoside Oryza sativa ?
-
 ?
laminaritriose + H2O 7.43% activity compared to n-octyl beta-D-glucopyranoside Oryza sativa ?
-
 ?
additional information the enzyme is not able to hydrolyze n-octyl beta-D-thioglucopyranoside, amygdalin, dalcochinin glucoside, gossypin, linamarin, naringin, phlorizin, prunasin, pyridoxine-5’-O-beta-D-glucoside, and quercitin 3-beta-D-glucoside. Negligible hydrolysis of 4-nitrophenyl-alpha-L-arabinoside, 4-nitrophenyl-beta-D-cellobioside, 4-nitrophenyl-beta-D-xyloside, 4-nitrophenyl-beta-D-mannoside, 4-nitrophenyl-beta-D-glucuronide, and 4-nitrophenyl-alpha-D-glucoside, cellotriose, cellotetraose, cellopentaose, cellohexaose, laminaritetraose, laminaripentaose, apigenin 7-glucoside, glycitin, diadzin, genistin, esculin, arbutin, coumaryl alcohol beta-D-glucoside, coniferin, and salicin Oryza sativa ?
-
 ?
n-heptyl-beta-D-glucoside + H2O 29.5% activity compared to n-octyl beta-D-glucopyranoside Oryza sativa ?
-
 ?
n-octyl beta-D-glucopyranoside + H2O 100% activity Oryza sativa ?
-
 ?
sophorose + H2O beta-(1-2)-linked sophorose, 31.9% activity compared to n-octyl beta-D-glucopyranoside Oryza sativa ?
-
 ?

Synonyms

Synonyms Comment Organism
3BGlu6
-
 Oryza sativa
beta-glucosidase 6
-
 Oryza sativa
BGlu1
-
 Oryza sativa
GH1 beta-glucosidase
-
 Oryza sativa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 55
-
 Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 the enzyme is most stable at 20°C to 30°C, and begins to lose activity after 40 min at 40°C or higher Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 cellobiose beta-(1-4)-linked cellobiose,in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
0.17
-
 gentiobiose beta-(1-6)-linked gentiobiose, in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
1.64
-
 laminaritriose in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
4.25
-
 n-heptyl-beta-D-glucoside in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
6.17
-
 laminaribiose beta-(1-3)-linked laminaribose,in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
9.8
-
 sophorose beta-(1-2)-linked sophorose, in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
9.8
-
 4-nitrophenyl-beta-D-galactopyranoside in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
33.5
-
 4-nitrophenyl-beta-D-fucopyranoside in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
38.9
-
 4-nitrophenyl-beta-D-glucopyranoside in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5
-
 Oryza sativa

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 7 the activity drops by 50% at pH 2.5 and 6.0, and is negligible from pH 7.0 upward Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0085
-
 cellobiose beta-(1-4)-linked cellobiose, in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
0.011
-
 gentiobiose beta-(1-6)-linked gentiobiose, in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
0.18
-
 laminaritriose in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
0.85
-
 n-heptyl-beta-D-glucoside in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
0.96
-
 sophorose beta-(1-2)-linked sophorose, in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
1.6
-
 4-nitrophenyl-beta-D-galactopyranoside in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
1.7
-
 laminaribiose beta-(1-3)-linked laminaribose, in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
6.2
-
 4-nitrophenyl-beta-D-glucopyranoside in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa
67
-
 4-nitrophenyl-beta-D-fucopyranoside in 100 mM sodium acetate, pH 5.0, at 30°C Oryza sativa