Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-glucose | D-glucose (1% w/v) in the reaction medium stimulates beta-glucosidase activity by about 2fold in crude extracts from mycelia grown in sugarcane bagasse | [Humicola] grisea var. thermoidea | |
D-xylose | xylose (1% w/v) in the reaction medium stimulates beta-glucosidase activity by about 2fold in crude extracts from mycelia grown in sugarcane bagasse | [Humicola] grisea var. thermoidea |
Application | Comment | Organism |
---|---|---|
biotechnology | the beta-glucosidase has a potential for biotechnological applications in the bioconversion of lignocellulosic materials | [Humicola] grisea var. thermoidea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
4-nitrophenyl-beta-D-fucopyranoside | at 50°C, in 50 mM sodium phosphate buffer, pH 6.0 | [Humicola] grisea var. thermoidea | |
0.12 | - |
4-nitrophenyl-beta-D-glucopyranoside | at 50°C, in 50 mM sodium phosphate buffer, pH 6.0 | [Humicola] grisea var. thermoidea | |
0.27 | - |
cellobiose | at 50°C, in 50 mM sodium phosphate buffer, pH 6.0 | [Humicola] grisea var. thermoidea | |
1.32 | - |
4-nitrophenyl-beta-D-galactopyranoside | at 50°C, in 50 mM sodium phosphate buffer, pH 6.0 | [Humicola] grisea var. thermoidea | |
1.42 | - |
lactose | at 50°C, in 50 mM sodium phosphate buffer, pH 6.0 | [Humicola] grisea var. thermoidea | |
6.8 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 50°C, in 50 mM sodium phosphate buffer, pH 6.0 | [Humicola] grisea var. thermoidea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
SDS-PAGE | [Humicola] grisea var. thermoidea |
60000 | - |
gel filtration | [Humicola] grisea var. thermoidea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | [Humicola] grisea var. thermoidea | best natural substrate | 2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Humicola] grisea var. thermoidea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Sephadex G-200 gel filtration, and DEAE-cellulose column chromatography | [Humicola] grisea var. thermoidea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
[Humicola] grisea var. thermoidea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.95 | - |
crude extract, at 50°C, in 50 mM sodium phosphate buffer, pH 6.0, using 4-nitrophenyl-beta-D-glucopyranoside as substrate | [Humicola] grisea var. thermoidea |
32.39 | - |
after 11fold purification, at 50°C, in 50 mM sodium phosphate buffer, pH 6.0, using 4-nitrophenyl-beta-D-glucopyranoside as substrate | [Humicola] grisea var. thermoidea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
[Humicola] grisea var. thermoidea | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | best synthetic substrate | [Humicola] grisea var. thermoidea | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
[Humicola] grisea var. thermoidea | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | highest specific activity | [Humicola] grisea var. thermoidea | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | lowest specific activity | [Humicola] grisea var. thermoidea | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
cellobiose + H2O | best natural substrate | [Humicola] grisea var. thermoidea | 2 beta-D-glucose | - |
? | |
lactose + H2O | - |
[Humicola] grisea var. thermoidea | D-glucose + D-galactose | - |
? | |
additional information | does not hydrolyze 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, avicel carboxymethyl cellulose , xylan, starch, and pectin | [Humicola] grisea var. thermoidea | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1,4-beta-glucosidase | - |
[Humicola] grisea var. thermoidea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
[Humicola] grisea var. thermoidea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | the purified enzyme is thermostable up to 60 min in water at 55°C and shows half-lives of 7 and 14 min when incubated in the absence or presence of 50 mM D-glucose, respectively, at 60°C | [Humicola] grisea var. thermoidea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
[Humicola] grisea var. thermoidea |
Organism | Comment | Expression |
---|---|---|
[Humicola] grisea var. thermoidea | the highest levels of total activity are obtained in liquid media supplemented with avicel or carboxymethyl cellulose | up |