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Literature summary for 3.2.1.21 extracted from

  • Nascimento, C.V.; Souza, F.H.; Masui, D.C.; Leone, F.A.; Peralta, R.M.; Jorge, J.A.; Furriel, R.P.
    Purification and biochemical properties of a glucose-stimulated beta-D-glucosidase produced by Humicola grisea var. thermoidea grown on sugarcane bagasse (2010), J. Microbiol., 48, 53-62.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-glucose D-glucose (1% w/v) in the reaction medium stimulates beta-glucosidase activity by about 2fold in crude extracts from mycelia grown in sugarcane bagasse [Humicola] grisea var. thermoidea
D-xylose xylose (1% w/v) in the reaction medium stimulates beta-glucosidase activity by about 2fold in crude extracts from mycelia grown in sugarcane bagasse [Humicola] grisea var. thermoidea

Application

Application Comment Organism
biotechnology the beta-glucosidase has a potential for biotechnological applications in the bioconversion of lignocellulosic materials [Humicola] grisea var. thermoidea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
4-nitrophenyl-beta-D-fucopyranoside at 50°C, in 50 mM sodium phosphate buffer, pH 6.0 [Humicola] grisea var. thermoidea
0.12
-
4-nitrophenyl-beta-D-glucopyranoside at 50°C, in 50 mM sodium phosphate buffer, pH 6.0 [Humicola] grisea var. thermoidea
0.27
-
cellobiose at 50°C, in 50 mM sodium phosphate buffer, pH 6.0 [Humicola] grisea var. thermoidea
1.32
-
4-nitrophenyl-beta-D-galactopyranoside at 50°C, in 50 mM sodium phosphate buffer, pH 6.0 [Humicola] grisea var. thermoidea
1.42
-
lactose at 50°C, in 50 mM sodium phosphate buffer, pH 6.0 [Humicola] grisea var. thermoidea
6.8
-
2-nitrophenyl-beta-D-galactopyranoside at 50°C, in 50 mM sodium phosphate buffer, pH 6.0 [Humicola] grisea var. thermoidea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
SDS-PAGE [Humicola] grisea var. thermoidea
60000
-
gel filtration [Humicola] grisea var. thermoidea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + H2O [Humicola] grisea var. thermoidea best natural substrate 2 beta-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
[Humicola] grisea var. thermoidea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephadex G-200 gel filtration, and DEAE-cellulose column chromatography [Humicola] grisea var. thermoidea

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
[Humicola] grisea var. thermoidea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.95
-
crude extract, at 50°C, in 50 mM sodium phosphate buffer, pH 6.0, using 4-nitrophenyl-beta-D-glucopyranoside as substrate [Humicola] grisea var. thermoidea
32.39
-
after 11fold purification, at 50°C, in 50 mM sodium phosphate buffer, pH 6.0, using 4-nitrophenyl-beta-D-glucopyranoside as substrate [Humicola] grisea var. thermoidea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
[Humicola] grisea var. thermoidea 2-nitrophenol + beta-D-galactopyranose
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O best synthetic substrate [Humicola] grisea var. thermoidea 4-nitrophenol + beta-D-fucopyranose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
[Humicola] grisea var. thermoidea 4-nitrophenol + beta-D-galactopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O highest specific activity [Humicola] grisea var. thermoidea 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O lowest specific activity [Humicola] grisea var. thermoidea 4-nitrophenol + beta-D-xylopyranose
-
?
cellobiose + H2O best natural substrate [Humicola] grisea var. thermoidea 2 beta-D-glucose
-
?
lactose + H2O
-
[Humicola] grisea var. thermoidea D-glucose + D-galactose
-
?
additional information does not hydrolyze 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, avicel carboxymethyl cellulose , xylan, starch, and pectin [Humicola] grisea var. thermoidea ?
-
?

Synonyms

Synonyms Comment Organism
1,4-beta-glucosidase
-
[Humicola] grisea var. thermoidea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
[Humicola] grisea var. thermoidea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 60 the purified enzyme is thermostable up to 60 min in water at 55°C and shows half-lives of 7 and 14 min when incubated in the absence or presence of 50 mM D-glucose, respectively, at 60°C [Humicola] grisea var. thermoidea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
[Humicola] grisea var. thermoidea

Expression

Organism Comment Expression
[Humicola] grisea var. thermoidea the highest levels of total activity are obtained in liquid media supplemented with avicel or carboxymethyl cellulose up