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Literature summary for 3.2.1.21 extracted from

  • Korotkova, O.G.; Semenova, M.V.; Morozova, V.V.; Zorov, I.N.; Sokolova, L.M.; Bubnova, T.M.; Okunev, O.N.; Sinitsyn, A.P.
    Isolation and properties of fungal beta-glucosidases (2009), Biochemistry, 74, 569-577.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
D-glucose competitive inhibition Aspergillus japonicus
D-glucose competitive inhibition Talaromyces verruculosus
D-glucose competitive inhibition Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 4-nitrophenyl-beta-D-glucopyranoside at pH 5.0 and 40°C Trichoderma reesei
0.44
-
 4-nitrophenyl-beta-D-glucopyranoside at pH 5.0 and 40°C Talaromyces verruculosus
0.6
-
 4-nitrophenyl-beta-D-glucopyranoside at pH 5.0 and 40°C Aspergillus japonicus
0.75
-
 cellobiose at pH 5.0 and 40°C Trichoderma reesei
0.95
-
 cellobiose at pH 5.0 and 40°C Aspergillus japonicus
1.2
-
 cellobiose at pH 5.0 and 40°C Talaromyces verruculosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
 SDS-PAGE Talaromyces verruculosus
120000
-
 SDS-PAGE Aspergillus japonicus

Organism

Organism UniProt Comment Textmining
Aspergillus japonicus
-
-
-
Talaromyces verruculosus
-
-
-
Trichoderma reesei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Source 15Q column chromatography, Biogel P4 gel filtration, and phenyl-Superose column chromatography Aspergillus japonicus
ammonium sulfate precipitation, Source 15Q column chromatography, Biogel P4 gel filtration, and Source 15 isopropyl column chromatography Trichoderma reesei
ammonium sulfate precipitation, Source 15Q column chromatography, Biogel P4 gel filtration, and Source 15 isopropyl column chromatography Talaromyces verruculosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
 using carboxymethylcellulose as substrate, at pH 5.0 and 50°C Aspergillus japonicus
3
-
 using carboxymethylcellulose as substrate, at pH 5.0 and 50°C Talaromyces verruculosus
3 8 using alpha-sophorose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
7
-
 using beta-gentiobiose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
9
-
 using beta-gentiobiose as substrate, at pH 5.0 and 40°C Trichoderma reesei
9
-
 using beta-glucan as substrate, at pH 5.0 and 50°C Aspergillus japonicus
9
-
 using carboxymethylcellulose as substrate, at pH 5.0 and 50°C Trichoderma reesei
17
-
 using laminaritriose as substrate, at pH 5.0 and 40°C Trichoderma reesei
18
-
 using cellohexaose as substrate, at pH 5.0 and 40°C Trichoderma reesei
18
-
 using cellopentaose as substrate, at pH 5.0 and 40°C Trichoderma reesei
19
-
 using cellotetraose as substrate, at pH 5.0 and 40°C Trichoderma reesei
20
-
 using cellotriose as substrate, at pH 5.0 and 40°C Trichoderma reesei
21
-
 using cellobiose as substrate, at pH 5.0 and 40°C Trichoderma reesei
23
-
 using alpha-sophorose as substrate, at pH 5.0 and 40°C Trichoderma reesei
32
-
 using laminarin as substrate, at pH 5.0 and 50°C Aspergillus japonicus
33
-
 using laminaribiose as substrate, at pH 5.0 and 40°C Trichoderma reesei
36
-
 using laminaritriose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
39
-
 using beta-glucan as substrate, at pH 5.0 and 50°C Trichoderma reesei
45
-
 using cellohexaose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
46
-
 using cellopentaose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
50
-
 using cellotetraose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
52
-
 using beta-glucan as substrate, at pH 5.0 and 50°C Talaromyces verruculosus
54
-
 using cellobiose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
54
-
 using cellopentaose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
55
-
 using cellohexaose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
55
-
 using cellotetraose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
56
-
 using cellotriose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
57
-
 using laminaribiose as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
63
-
 using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 5.0 and 40°C Trichoderma reesei
69
-
 using cellotriose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
72
-
 using alpha-sophorose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
81
-
 using laminarin as substrate, at pH 5.0 and 50°C Trichoderma reesei
83
-
 using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 5.0 and 40°C Talaromyces verruculosus
87
-
 using laminaritriose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
102
-
 using laminarin as substrate, at pH 5.0 and 50°C Talaromyces verruculosus
105
-
 using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 5.0 and 40°C Aspergillus japonicus
124
-
 using cellobiose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
147
-
 using beta-gentiobiose as substrate, at pH 5.0 and 40°C Aspergillus japonicus
169
-
 using laminaribiose as substrate, at pH 5.0 and 40°C Aspergillus japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus japonicus 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O low activity Trichoderma reesei 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O low activity Talaromyces verruculosus 4-nitrophenol + D-glucopyranose
-
 ?
alpha-sophorose + H2O
-
 Trichoderma reesei beta-D-glucose
-
 ?
alpha-sophorose + H2O
-
 Aspergillus japonicus beta-D-glucose
-
 ?
alpha-sophorose + H2O
-
 Talaromyces verruculosus beta-D-glucose
-
 ?
beta-gentiobiose + H2O
-
 Trichoderma reesei beta-D-glucose
-
 ?
beta-gentiobiose + H2O
-
 Aspergillus japonicus beta-D-glucose
-
 ?
beta-gentiobiose + H2O
-
 Talaromyces verruculosus beta-D-glucose
-
 ?
beta-glucan + H2O
-
 Trichoderma reesei ?
-
 ?
beta-glucan + H2O
-
 Talaromyces verruculosus ?
-
 ?
beta-glucan + H2O low activity Aspergillus japonicus ?
-
 ?
cellobiose + H2O low activity, the specific activity towards cellobiose is lower than towards 4-nitrophenyl-beta-D-glucopyranoside Trichoderma reesei 2 beta-D-glucose
-
 ?
cellobiose + H2O low activity, the specific activity towards cellobiose is lower than towards 4-nitrophenyl-beta-D-glucopyranoside Talaromyces verruculosus 2 beta-D-glucose
-
 ?
cellobiose + H2O the specific activity towards cellobiose is higher than towards 4-nitrophenyl-beta-D-glucopyranoside Aspergillus japonicus 2 beta-D-glucose
-
 ?
cellohexaose + H2O
-
 Trichoderma reesei beta-D-glucose
-
 ?
cellohexaose + H2O
-
 Aspergillus japonicus beta-D-glucose
-
 ?
cellohexaose + H2O
-
 Talaromyces verruculosus beta-D-glucose
-
 ?
cellopentaose + H2O
-
 Trichoderma reesei 5 D-glucose
-
 ?
cellopentaose + H2O
-
 Aspergillus japonicus 5 D-glucose
-
 ?
cellopentaose + H2O
-
 Talaromyces verruculosus 5 D-glucose
-
 ?
cellotetraose + 3 H2O
-
 Trichoderma reesei 4 beta-D-glucose
-
 ?
cellotetraose + 3 H2O
-
 Aspergillus japonicus 4 beta-D-glucose
-
 ?
cellotetraose + 3 H2O
-
 Talaromyces verruculosus 4 beta-D-glucose
-
 ?
cellotriose + H2O
-
 Trichoderma reesei 3 D-glucose
-
 ?
cellotriose + H2O
-
 Aspergillus japonicus 3 D-glucose
-
 ?
cellotriose + H2O
-
 Talaromyces verruculosus 3 D-glucose
-
 ?
laminaribiose + H2O
-
 Trichoderma reesei 2 beta-D-glucose
-
 ?
laminaribiose + H2O
-
 Aspergillus japonicus 2 beta-D-glucose
-
 ?
laminaribiose + H2O
-
 Talaromyces verruculosus 2 beta-D-glucose
-
 ?
laminarin + H2O low activity Aspergillus japonicus beta-D-glucose + ?
-
 ?
laminarin + H2O highest specific activity Trichoderma reesei beta-D-glucose + ?
-
 ?
laminarin + H2O highest specific activity Talaromyces verruculosus beta-D-glucose + ?
-
 ?
laminaritriose + 2 H2O
-
 Trichoderma reesei 3 beta-D-glucose
-
 ?
laminaritriose + 2 H2O
-
 Aspergillus japonicus 3 beta-D-glucose
-
 ?
laminaritriose + 2 H2O
-
 Talaromyces verruculosus 3 beta-D-glucose
-
 ?
additional information no activity towards carboxymethylcellulose Trichoderma reesei ?
-
 ?
additional information no activity towards carboxymethylcellulose Aspergillus japonicus ?
-
 ?
additional information no activity towards carboxymethylcellulose Talaromyces verruculosus ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-D-glucoside glucohydrolase
-
 Trichoderma reesei
beta-D-glucoside glucohydrolase
-
 Aspergillus japonicus
beta-D-glucoside glucohydrolase
-
 Talaromyces verruculosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 at pH 5.0 Talaromyces verruculosus
65
-
 at pH 5.0 Aspergillus japonicus
70
-
 at pH 5.0 Trichoderma reesei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80
-
 Talaromyces verruculosus
48 72
-
 Aspergillus japonicus
50 80
-
 Trichoderma reesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 stable at 30°C retaining 90% of the original activity for more than 24 h, after 3 h of incubation at 40°C the enzyme loses 70-85% of its activity, after 1 h of incubation at 50°C the enzyme retains 20% of its activity Talaromyces verruculosus
30 40 stable at 30°C retaining 90% of the original activity for more than 24 h, after 3 h of incubation at 40°C the enzyme loses 70-85% of its activity, after 1 h of incubation at 50°C the enzyme retains 43% of its activity Trichoderma reesei
30 40 stable at 30°C retaining 90% of the original activity for more than 24 h, after 3 h of incubation at 40°C the enzyme retains 50% of its activity, after 1 h of incubation at 50°C the enzyme retains 57% of its activity Aspergillus japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
42
-
 cellobiose at pH 5.0 and 40°C Trichoderma reesei
89
-
 cellobiose at pH 5.0 and 40°C Talaromyces verruculosus
118
-
 4-nitrophenyl-beta-D-glucopyranoside at pH 5.0 and 40°C Trichoderma reesei
160
-
 4-nitrophenyl-beta-D-glucopyranoside at pH 5.0 and 40°C Talaromyces verruculosus
259
-
 4-nitrophenyl-beta-D-glucopyranoside at pH 5.0 and 40°C Aspergillus japonicus
350
-
 cellobiose at pH 5.0 and 40°C Aspergillus japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5 at 40°C Aspergillus japonicus
5
-
 at 40°C Talaromyces verruculosus
5.5
-
 at 40°C Trichoderma reesei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.51
-
 D-glucose at pH 5.0 and 40°C Trichoderma reesei
0.93
-
 D-glucose at pH 5.0 and 40°C Talaromyces verruculosus
2.73
-
 D-glucose at pH 5.0 and 40°C Aspergillus japonicus