Protein Variants | Comment | Organism |
---|---|---|
F193A | increase in affinity for a small polar aglycone, deep decrease in kcat value. 7.8% of wild-type activity for substrate 4-nitrophenyl-beta-D-glucoside, 3% for substrate 4-methylumbelliferyl-beta-D-glucoside, respectively | Zea mays |
F193A/F200K/W373K/F461L | almost complete loss of catalytic activity, extensive alterations in strucutre | Zea mays |
F200K | 1.1% of wild-type activity for substrate 4-nitrophenyl-beta-D-glucoside, 0.3% for substrate 4-methylumbelliferyl-beta-D-glucoside, respectively | Zea mays |
F461L | 120% of wild-type activity for substrate 4-nitrophenyl-beta-D-glucoside, 119% for substrate 4-methylumbelliferyl-beta-D-glucoside, respectively | Zea mays |
W373K | large decrease in kcat value. 0.5% of wild-type activity for substrate 4-nitrophenyl-beta-D-glucoside, 0.04% for substrate 4-methylumbelliferyl-beta-D-glucoside, respectively | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
4-nitrophenyl-beta-D-glucoside | mutant F193A | Zea mays | |
0.12 | - |
4-methylumbelliferyl-beta-D-glucoside | mutant F193A | Zea mays | |
0.148 | - |
4-methylumbelliferyl-beta-D-glucoside | wild-type | Zea mays | |
0.164 | - |
4-methylumbelliferyl-beta-D-glucoside | mutant F461L | Zea mays | |
0.65 | - |
4-nitrophenyl-beta-D-glucoside | mutant F461L | Zea mays | |
0.68 | - |
4-nitrophenyl-beta-D-glucoside | wild-type | Zea mays | |
1.51 | - |
4-methylumbelliferyl-beta-D-glucoside | mutant F200K | Zea mays | |
1.73 | - |
4-methylumbelliferyl-beta-D-glucoside | mutant W373K | Zea mays | |
2.1 | - |
4-nitrophenyl-beta-D-glucoside | mutant W373K | Zea mays | |
3.5 | - |
4-nitrophenyl-beta-D-glucoside | mutant F200K | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | P49235 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-glucoside + H2O | - |
Zea mays | 4-methylumbelliferone + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Zea mays | 4-nitrophenol + D-glucopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ZM-p60.1 | - |
Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
4-nitrophenyl-beta-D-glucoside | mutant F193A | Zea mays | |
0.22 | - |
4-methylumbelliferyl-beta-D-glucoside | mutant W373K | Zea mays | |
0.63 | - |
4-nitrophenyl-beta-D-glucoside | mutant W373K | Zea mays | |
1.29 | - |
4-methylumbelliferyl-beta-D-glucoside | mutant F193A | Zea mays | |
1.87 | - |
4-methylumbelliferyl-beta-D-glucoside | mutant F200K | Zea mays | |
2.43 | - |
4-nitrophenyl-beta-D-glucoside | mutant F200K | Zea mays | |
42.8 | - |
4-nitrophenyl-beta-D-glucoside | wild-type | Zea mays | |
49.27 | - |
4-nitrophenyl-beta-D-glucoside | mutant F461L | Zea mays | |
53.8 | - |
4-methylumbelliferyl-beta-D-glucoside | wild-type | Zea mays | |
70.88 | - |
4-methylumbelliferyl-beta-D-glucoside | mutant F461L | Zea mays |