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Literature summary for 3.2.1.21 extracted from

  • Dopitova, R.; Mazura, P.; Janda, L.; Chaloupkova, R.; Jerabek, P.; Damborsky, J.; Filipi, T.; Kiran, N.S.; Brzobohaty, B.
    Functional analysis of the aglycone-binding site of the maize beta-glucosidase Zm-p60.1 (2008), FEBS J., 275, 6123-6135.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
F193A increase in affinity for a small polar aglycone, deep decrease in kcat value. 7.8% of wild-type activity for substrate 4-nitrophenyl-beta-D-glucoside, 3% for substrate 4-methylumbelliferyl-beta-D-glucoside, respectively Zea mays
F193A/F200K/W373K/F461L almost complete loss of catalytic activity, extensive alterations in strucutre Zea mays
F200K 1.1% of wild-type activity for substrate 4-nitrophenyl-beta-D-glucoside, 0.3% for substrate 4-methylumbelliferyl-beta-D-glucoside, respectively Zea mays
F461L 120% of wild-type activity for substrate 4-nitrophenyl-beta-D-glucoside, 119% for substrate 4-methylumbelliferyl-beta-D-glucoside, respectively Zea mays
W373K large decrease in kcat value. 0.5% of wild-type activity for substrate 4-nitrophenyl-beta-D-glucoside, 0.04% for substrate 4-methylumbelliferyl-beta-D-glucoside, respectively Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
4-nitrophenyl-beta-D-glucoside mutant F193A Zea mays
0.12
-
4-methylumbelliferyl-beta-D-glucoside mutant F193A Zea mays
0.148
-
4-methylumbelliferyl-beta-D-glucoside wild-type Zea mays
0.164
-
4-methylumbelliferyl-beta-D-glucoside mutant F461L Zea mays
0.65
-
4-nitrophenyl-beta-D-glucoside mutant F461L Zea mays
0.68
-
4-nitrophenyl-beta-D-glucoside wild-type Zea mays
1.51
-
4-methylumbelliferyl-beta-D-glucoside mutant F200K Zea mays
1.73
-
4-methylumbelliferyl-beta-D-glucoside mutant W373K Zea mays
2.1
-
4-nitrophenyl-beta-D-glucoside mutant W373K Zea mays
3.5
-
4-nitrophenyl-beta-D-glucoside mutant F200K Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays P49235
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucoside + H2O
-
Zea mays 4-methylumbelliferone + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Zea mays 4-nitrophenol + D-glucopyranose
-
?

Synonyms

Synonyms Comment Organism
ZM-p60.1
-
Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
4-nitrophenyl-beta-D-glucoside mutant F193A Zea mays
0.22
-
4-methylumbelliferyl-beta-D-glucoside mutant W373K Zea mays
0.63
-
4-nitrophenyl-beta-D-glucoside mutant W373K Zea mays
1.29
-
4-methylumbelliferyl-beta-D-glucoside mutant F193A Zea mays
1.87
-
4-methylumbelliferyl-beta-D-glucoside mutant F200K Zea mays
2.43
-
4-nitrophenyl-beta-D-glucoside mutant F200K Zea mays
42.8
-
4-nitrophenyl-beta-D-glucoside wild-type Zea mays
49.27
-
4-nitrophenyl-beta-D-glucoside mutant F461L Zea mays
53.8
-
4-methylumbelliferyl-beta-D-glucoside wild-type Zea mays
70.88
-
4-methylumbelliferyl-beta-D-glucoside mutant F461L Zea mays