Cloned (Comment) | Organism |
---|---|
- |
Spodoptera frugiperda |
Protein Variants | Comment | Organism |
---|---|---|
E190Q | mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. E190 favors the binding of the initial portion of alkyl-type aglycones | Spodoptera frugiperda |
E194A | mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. E190 favors the binding of the initial portion of alkyl-type aglycones | Spodoptera frugiperda |
K201A | mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. K201 is relevant for binding of D-glucose units | Spodoptera frugiperda |
K201F | mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. K201 is relevant for binding of D-glucose units | Spodoptera frugiperda |
M453A | mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. M453 favors the binding of the second D-glucose unit of oligosaccharidic aglycones | Spodoptera frugiperda |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | - |
Spodoptera frugiperda | |
cellopentaose | - |
Spodoptera frugiperda | |
cellotetraose | - |
Spodoptera frugiperda | |
cellotriose | - |
Spodoptera frugiperda | |
heptyl beta-D-glucoside | - |
Spodoptera frugiperda | |
hexyl beta-D-glucoside | - |
Spodoptera frugiperda | |
methyl beta-D-glucoside | - |
Spodoptera frugiperda | |
nonyl beta-D-glucoside | - |
Spodoptera frugiperda | |
octyl beta-D-glucoside | - |
Spodoptera frugiperda | |
phenyl beta-D-glucoside | - |
Spodoptera frugiperda |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
4-methylumbeliferyl beta-D-glucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.38 | - |
4-nitrophenyl beta-D-fucoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.59 | - |
4-nitrophenyl beta-D-fucoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.62 | - |
4-nitrophenyl beta-D-fucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.65 | - |
4-nitrophenyl beta-D-fucoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.66 | - |
4-nitrophenyl beta-D-fucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.67 | - |
4-nitrophenyl beta-D-fucoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.71 | - |
4-nitrophenyl beta-D-fucoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.9 | - |
4-nitrophenyl beta-D-glucoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.04 | - |
4-nitrophenyl beta-D-xyloside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.05 | - |
4-nitrophenyl beta-D-glucoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.2 | - |
4-nitrophenyl beta-D-glucoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.2 | - |
4-nitrophenyl beta-D-glucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.26 | - |
4-nitrophenyl beta-D-glucoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.56 | - |
4-nitrophenyl beta-D-xyloside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.7 | - |
4-methylumbeliferyl beta-D-glucoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.1 | - |
4-nitrophenyl beta-D-glucoside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.3 | - |
4-methylumbeliferyl beta-D-glucoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.4 | - |
4-nitrophenyl beta-D-xyloside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.6 | - |
4-methylumbeliferyl beta-D-glucoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.6 | - |
4-nitrophenyl beta-D-glucoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.6 | - |
4-methylumbeliferyl beta-D-glucoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.9 | - |
4-nitrophenyl beta-D-galactoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
3.6 | - |
4-nitrophenyl beta-D-xyloside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
4.4 | - |
4-nitrophenyl beta-D-galactoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
4.7 | - |
4-nitrophenyl beta-D-xyloside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
4.9 | - |
4-nitrophenyl beta-D-xyloside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
4.9 | - |
4-nitrophenyl beta-D-xyloside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
4.9 | - |
4-nitrophenyl beta-D-galactoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
6.2 | - |
4-methylumbeliferyl beta-D-glucoside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
6.2 | - |
4-nitrophenyl beta-D-galactoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
6.3 | - |
4-methylumbeliferyl beta-D-glucoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
8.2 | - |
4-nitrophenyl beta-D-galactoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
9 | - |
4-nitrophenyl beta-D-galactoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
9.9 | - |
4-nitrophenyl beta-D-galactoside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spodoptera frugiperda | O61594 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbeliferyl beta-D-glucoside + H2O | - |
Spodoptera frugiperda | 4-methylumbelliferol + D-glucose | - |
? | |
4-nitrophenyl beta-D-fucoside + H2O | - |
Spodoptera frugiperda | 4-nitrophenol + D-fucose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | - |
Spodoptera frugiperda | 4-nitrophenol + D-galactose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Spodoptera frugiperda | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-xyloside + H2O | - |
Spodoptera frugiperda | 4-nitrophenol + D-xylose | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00188 | - |
4-nitrophenyl beta-D-xyloside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.0073 | - |
4-nitrophenyl beta-D-xyloside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.0127 | - |
4-nitrophenyl beta-D-xyloside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.02 | - |
4-methylumbeliferyl beta-D-glucoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.0211 | - |
4-nitrophenyl beta-D-xyloside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.0238 | - |
4-nitrophenyl beta-D-xyloside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.0302 | - |
4-nitrophenyl beta-D-xyloside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.032 | - |
4-methylumbeliferyl beta-D-glucoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.058 | - |
4-nitrophenyl beta-D-galactoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.072 | - |
4-nitrophenyl beta-D-galactoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.089 | - |
4-nitrophenyl beta-D-xyloside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.114 | - |
4-methylumbeliferyl beta-D-glucoside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.153 | - |
4-nitrophenyl beta-D-galactoside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.174 | - |
4-nitrophenyl beta-D-galactoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.189 | - |
4-nitrophenyl beta-D-galactoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.231 | - |
4-methylumbeliferyl beta-D-glucoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.2674 | - |
4-methylumbeliferyl beta-D-glucoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.269 | - |
4-nitrophenyl beta-D-galactoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.348 | - |
4-nitrophenyl beta-D-fucoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.56 | - |
4-nitrophenyl beta-D-glucoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.59 | - |
4-nitrophenyl beta-D-glucoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.62 | - |
4-methylumbeliferyl beta-D-glucoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.65 | - |
4-nitrophenyl beta-D-glucoside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.66 | - |
4-nitrophenyl beta-D-glucoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.668 | - |
4-nitrophenyl beta-D-fucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.98 | - |
4-nitrophenyl beta-D-fucoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.32 | - |
4-methylumbeliferyl beta-D-glucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.52 | - |
4-nitrophenyl beta-D-glucoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.26 | - |
4-nitrophenyl beta-D-glucoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.43 | - |
4-nitrophenyl beta-D-fucoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
4 | - |
4-nitrophenyl beta-D-glucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
4.03 | - |
4-nitrophenyl beta-D-galactoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
4.15 | - |
4-nitrophenyl beta-D-fucoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
7.14 | - |
4-nitrophenyl beta-D-fucoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
8.9 | - |
4-nitrophenyl beta-D-fucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
cellotriose | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.07 | - |
cellotriose | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.09 | - |
cellotriose | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.13 | - |
nonyl beta-D-glucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.14 | - |
cellotetraose | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.2 | - |
cellopentaose | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.2 | - |
cellotetraose | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.21 | - |
cellotetraose | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.22 | - |
nonyl beta-D-glucoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.25 | - |
cellotetraose | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.25 | - |
cellopentaose | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.26 | - |
cellopentaose | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.27 | - |
cellotriose | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.3 | - |
cellopentaose | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.41 | - |
cellotriose | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.42 | - |
octyl beta-D-glucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.44 | - |
octyl beta-D-glucoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.5 | - |
nonyl beta-D-glucoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.54 | - |
cellotetraose | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.7 | - |
heptyl beta-D-glucoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
0.83 | - |
nonyl beta-D-glucoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.12 | - |
heptyl beta-D-glucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.16 | - |
nonyl beta-D-glucoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.2 | - |
cellobiose | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.3 | - |
octyl beta-D-glucoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.49 | - |
octyl beta-D-glucoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
1.57 | - |
hexyl beta-D-glucoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
2 | - |
cellopentaose | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.3 | - |
cellotriose | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.3 | - |
cellopentaose | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.4 | - |
cellotetraose | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.52 | - |
octyl beta-D-glucoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.6 | - |
heptyl beta-D-glucoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
2.9 | - |
cellobiose | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
3.3 | - |
hexyl beta-D-glucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
3.6 | - |
heptyl beta-D-glucoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
4.2 | - |
heptyl beta-D-glucoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
5.1 | - |
methyl beta-D-glucoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
5.2 | - |
cellobiose | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
5.6 | - |
hexyl beta-D-glucoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
5.7 | - |
nonyl beta-D-glucoside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
5.9 | - |
nonyl beta-D-glucoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
8 | - |
methyl beta-D-glucoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
8 | - |
hexyl beta-D-glucoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
8.6 | - |
cellotriose | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
10 | - |
hexyl beta-D-glucoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
10 | - |
methyl beta-D-glucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
10.1 | - |
methyl beta-D-glucoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
10.2 | - |
methyl beta-D-glucoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
10.3 | - |
octyl beta-D-glucoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
11 | - |
octyl beta-D-glucoside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
12.1 | - |
methyl beta-D-glucoside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
13.7 | - |
cellobiose | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
15.6 | - |
methyl beta-D-glucoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
16.2 | - |
cellopentaose | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
17 | - |
cellobiose | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
19.6 | - |
cellotetraose | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
21 | - |
heptyl beta-D-glucoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
21.8 | - |
heptyl beta-D-glucoside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
30 | - |
phenyl beta-D-glucoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
30 | - |
cellobiose | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
32 | - |
hexyl beta-D-glucoside | mutant E190A, 30°C, pH 6.0 | Spodoptera frugiperda | |
36 | - |
phenyl beta-D-glucoside | mutant E194A, 30°C, pH 6.0 | Spodoptera frugiperda | |
44.9 | - |
hexyl beta-D-glucoside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
48 | - |
phenyl beta-D-glucoside | mutant K201F, 30°C, pH 6.0 | Spodoptera frugiperda | |
49 | - |
phenyl beta-D-glucoside | wild-type, 30°C, pH 6.0 | Spodoptera frugiperda | |
52 | - |
phenyl beta-D-glucoside | mutant M453A, 30°C, pH 6.0 | Spodoptera frugiperda | |
74 | - |
phenyl beta-D-glucoside | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda | |
78 | - |
phenyl beta-D-glucoside | mutant E190Q, 30°C, pH 6.0 | Spodoptera frugiperda | |
81 | - |
cellobiose | mutant K201A, 30°C, pH 6.0 | Spodoptera frugiperda |