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Literature summary for 3.2.1.21 extracted from

  • Kaya, M.; Ito, J.; Kotaka, A.; Matsumura, K.; Bando, H.; Sahara, H.; Ogino, C.; Shibasaki, S.; Kuroda, K.; Ueda, M.; Kondo, A.; Hata, Y.
    Isoflavone aglycones production from isoflavone glycosides by display of beta-glucosidase from Aspergillus oryzae on yeast cell surface (2008), Appl. Microbiol. Biotechnol., 79, 51-60.
    View publication on PubMed
Search for more literature for 3.2.1.21

Application

Application Comment Organism
biotechnology production of isoflavone aglycones by the enzyme. Isoform BGL1 shows broad substrate specificity to various isoflavone glycosides, the residual ratio is reached to 6.2% of the total amount of isoflavone glycosides and the hydrolysis reaction is almost finished within 48 h Aspergillus oryzae

Cloned(Commentary)

Cloned (Comment) Organism
expression as fusion protein with alpha-agglutinin on surface of Saccharomyces cerevisiae cells Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae Q2UIR4 isoform BGL1
-
Aspergillus oryzae Q2UL94
-
-
Aspergillus oryzae Q2UL94 isoform BGL3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O high activity Aspergillus oryzae 4-nitrophenol + D-glucopyranose
-
 ?
daidzein 7-O-(6-O-acetyl-beta-D-glucoside) + H2O
-
 Aspergillus oryzae daidzein + 6-O-acetyl-beta-D-glucose
-
 ?
daidzein-7-O-beta-D-glucopyranoside + H2O
-
 Aspergillus oryzae daidzein + beta-D-glucose
-
 ?
genistein-7-O-beta-D-glucopyranoside + H2O
-
 Aspergillus oryzae genistein + beta-D-glucose
-
 ?
genistein-7-O-beta-D-glucosyl-6''-O-acetate + H2O
-
 Aspergillus oryzae genistein + 6-O-acetyl-beta-D-glucose
-
 ?
glycitein-7-O-beta-D-glucopyranoside + H2O
-
 Aspergillus oryzae glycitein + beta-D-glucose
-
 ?
glycitein-7-O-beta-D-glucosyl-6''-O-acetate + H2O
-
 Aspergillus oryzae glycitein + 6-O-acetyl-beta-D-glucose
-
 ?
additional information low substrate specificity for isoflavones Aspergillus oryzae ?
-
 ?

Synonyms

Synonyms Comment Organism
Bgl3
-
 Aspergillus oryzae