Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition of piceid-beta-D-glucosidase | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.92 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 60°C | Aspergillus oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect on piceid-beta-D-glucosidase by Ca2+, Mg2+, and Zn2+ ions | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
about, piceid-beta-D-glucosidase, gel filtration | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
piceid + H2O | Aspergillus oryzae | - |
resveratrol + beta-D-glucose | - |
? | |
piceid + H2O | Aspergillus oryzae FFCDL-100 | - |
resveratrol + beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Aspergillus oryzae FFCDL-100 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native piceid-beta-D-glucosidase 12.6fold by ammonium sulfate fractionation, anion exchange chromatography, and freeze drying | Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.3 | - |
purified enzyme, substrate piceid | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | low activity | Aspergillus oryzae | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | low activity | Aspergillus oryzae FFCDL-100 | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | low activity | Aspergillus oryzae | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | low activity | Aspergillus oryzae FFCDL-100 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Aspergillus oryzae | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Aspergillus oryzae FFCDL-100 | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl beta-L-arabinopyranoside + H2O | low activity | Aspergillus oryzae | 4-nitrophenol + beta-L-arabinopyranose | - |
? | |
additional information | no activity with 4-nitrophenyl-alpha-L-arabinoside and 4-nitrophenyl-alpha-L-rhamnoside | Aspergillus oryzae | ? | - |
? | |
additional information | no activity with 4-nitrophenyl-alpha-L-arabinoside and 4-nitrophenyl-alpha-L-rhamnoside | Aspergillus oryzae FFCDL-100 | ? | - |
? | |
piceid + H2O | - |
Aspergillus oryzae | resveratrol + beta-D-glucose | - |
? | |
piceid + H2O | specific for, high activity | Aspergillus oryzae | resveratrol + beta-D-glucose | - |
? | |
piceid + H2O | - |
Aspergillus oryzae FFCDL-100 | resveratrol + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 77000, piceid-beta-D-glucosidase, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
piceid-beta-D-glucosidase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
piceid-beta-D-glucosidase | Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | temperature profile | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, purified piceid-beta-D-glucosidase, stable up to | Aspergillus oryzae |
60 | - |
30 min, purified piceid-beta-D-glucosidase, loss of 10% activity | Aspergillus oryzae |
70 | - |
purified piceid-beta-D-glucosidase, inactivation | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
piceid-beta-D-glucosidase | Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | pH profile | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | 30 min, purified piceid-beta-D-glucosidase, stable | Aspergillus oryzae |