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Literature summary for 3.2.1.21 extracted from

  • Suzuki, H.; Takahashi, S.; Watanabe, R.; Fukushima, Y.; Fujita, N.; Noguchi, A.; Yokoyama, R.; Nishitani, K.; Nishino, T.; Nakayama, T.
    An isoflavone conjugate-hydrolyzing beta-glucosidase from the roots of soybean (Glycine max) seedlings: purification, gene cloning, phylogenetics, and cellular localization (2006), J. Biol. Chem., 281, 30251-30259.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and anaylsis, expression analysis by semi-quantitative RT-PCR, stable expression of the 35S-GmICHG:GFP fusion gene in Arabidopsis thaliana T87 cells using an Agrobacterium tumefaciens GV3101(pMP90) transformation system, phylogenetic analysis Glycine max

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 89% inhibition at 0.1 mM Glycine max
additional information no or poor effects by 0.1 mM of Co2+, Cu2+, Mg2+, Mn2+, Ni2+, Zn2+, or EDTA Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
daidzein 7'-O-(6''-O-beta-D-malonyl-beta-D-glucoside) pH 7.0, 30°C Glycine max
0.025
-
genistein 7-O-(6''-O-malonyl-beta-D-glucoside) pH 7.0, 30°C Glycine max
0.025
-
daidzein 7'-O-beta-D-glucoside pH 7.0, 30°C Glycine max
0.032
-
genistein 7-O-beta-D-glucoside pH 7.0, 30°C Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall especially of root hairs Glycine max 5618
-
intercellular space of seedling roots Glycine max
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
2 * 58000, SDS-PAGE Glycine max
100000
-
gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Glycine max the enzyme is important in flavonoid-mediated interactions of legumes with microbes ?
-
?

Organism

Organism UniProt Comment Textmining
Glycine max Q08IT7
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Glycine max

Purification (Commentary)

Purification (Comment) Organism
native enzyme from roots, 4199fold by ammonium sulfate fractionation, anion and cation exchange chromatography, hydrophobic interaction chromatography, gel filtration, and heparin affinity chromatography, to homogeneity Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
additional information no activity in hypocotyl and cotyledons Glycine max
-
root
-
Glycine max
-
root hair
-
Glycine max
-
seedling
-
Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
660
-
purified native enzyme Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
daidzein 7'-O-(6''-O-beta-D-malonyl-beta-D-glucoside) + H2O
-
Glycine max daidzein + 6-O-malonyl-beta-D-glucopyranose
-
?
daidzein 7-O-(6-O-malonyl-beta-D-glucoside) + H2O
-
Glycine max daidzein + 6-O-malonyl-beta-D-glucopyranose
-
?
daidzein 7-O-beta-D-glucopyranoside + H2O
-
Glycine max daidzein + beta-D-glucose
-
?
genistein 7-O-(6''-O-malonyl-beta-D-glucoside) + H2O preferred substrate Glycine max genistein + 6-O-malonyl-beta-D-glucose
-
?
genistein 7-O-beta-D-glucoside + H2O low activity Glycine max genistein + beta-D-glucose
-
?
additional information the enzyme is important in flavonoid-mediated interactions of legumes with microbes Glycine max ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 58000, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
ICHG
-
Glycine max
isoflavone conjugate-hydrolyzing beta-glucosidase
-
Glycine max
More GmICHGis a member of glycoside hydrolase family 1 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13
-
genistein 7-O-beta-D-glucoside pH 7.0, 30°C Glycine max
22
-
daidzein 7'-O-beta-D-glucoside pH 7.0, 30°C Glycine max
46
-
daidzein 7'-O-(6''-O-beta-D-malonyl-beta-D-glucoside) pH 7.0, 30°C Glycine max
98
-
genistein 7-O-(6''-O-malonyl-beta-D-glucoside) pH 7.0, 30°C Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Glycine max