Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and anaylsis, expression analysis by semi-quantitative RT-PCR, stable expression of the 35S-GmICHG:GFP fusion gene in Arabidopsis thaliana T87 cells using an Agrobacterium tumefaciens GV3101(pMP90) transformation system, phylogenetic analysis | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 89% inhibition at 0.1 mM | Glycine max | |
additional information | no or poor effects by 0.1 mM of Co2+, Cu2+, Mg2+, Mn2+, Ni2+, Zn2+, or EDTA | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
daidzein 7'-O-(6''-O-beta-D-malonyl-beta-D-glucoside) | pH 7.0, 30°C | Glycine max | |
0.025 | - |
genistein 7-O-(6''-O-malonyl-beta-D-glucoside) | pH 7.0, 30°C | Glycine max | |
0.025 | - |
daidzein 7'-O-beta-D-glucoside | pH 7.0, 30°C | Glycine max | |
0.032 | - |
genistein 7-O-beta-D-glucoside | pH 7.0, 30°C | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | especially of root hairs | Glycine max | 5618 | - |
intercellular space | of seedling roots | Glycine max | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, SDS-PAGE | Glycine max |
100000 | - |
gel filtration | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Glycine max | the enzyme is important in flavonoid-mediated interactions of legumes with microbes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | Q08IT7 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Glycine max |
Purification (Comment) | Organism |
---|---|
native enzyme from roots, 4199fold by ammonium sulfate fractionation, anion and cation exchange chromatography, hydrophobic interaction chromatography, gel filtration, and heparin affinity chromatography, to homogeneity | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | no activity in hypocotyl and cotyledons | Glycine max | - |
root | - |
Glycine max | - |
root hair | - |
Glycine max | - |
seedling | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
660 | - |
purified native enzyme | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
daidzein 7'-O-(6''-O-beta-D-malonyl-beta-D-glucoside) + H2O | - |
Glycine max | daidzein + 6-O-malonyl-beta-D-glucopyranose | - |
? | |
daidzein 7-O-(6-O-malonyl-beta-D-glucoside) + H2O | - |
Glycine max | daidzein + 6-O-malonyl-beta-D-glucopyranose | - |
? | |
daidzein 7-O-beta-D-glucopyranoside + H2O | - |
Glycine max | daidzein + beta-D-glucose | - |
? | |
genistein 7-O-(6''-O-malonyl-beta-D-glucoside) + H2O | preferred substrate | Glycine max | genistein + 6-O-malonyl-beta-D-glucose | - |
? | |
genistein 7-O-beta-D-glucoside + H2O | low activity | Glycine max | genistein + beta-D-glucose | - |
? | |
additional information | the enzyme is important in flavonoid-mediated interactions of legumes with microbes | Glycine max | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, SDS-PAGE | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
ICHG | - |
Glycine max |
isoflavone conjugate-hydrolyzing beta-glucosidase | - |
Glycine max |
More | GmICHGis a member of glycoside hydrolase family 1 | Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
genistein 7-O-beta-D-glucoside | pH 7.0, 30°C | Glycine max | |
22 | - |
daidzein 7'-O-beta-D-glucoside | pH 7.0, 30°C | Glycine max | |
46 | - |
daidzein 7'-O-(6''-O-beta-D-malonyl-beta-D-glucoside) | pH 7.0, 30°C | Glycine max | |
98 | - |
genistein 7-O-(6''-O-malonyl-beta-D-glucoside) | pH 7.0, 30°C | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Glycine max |