Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Kim, B.; Singh, S.P.; Hayashi, K.
    Characteristics of chimeric enzymes constructed between Thermotoga maritima and Agrobacterium tumefaciens beta-glucosidases: role of C-terminal domain in catalytic activity (2006), Enzyme Microb. Technol., 38, 952-959.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene bglb, DNA and amino acid sequence determination and anaylsis, expression of N-terminally His-tagged wild-type and chimeric mutant enzymes in Escherichia coli Thermotoga maritima
gene cbg1, DNA and amino acid sequence determination and anaylsis, expression of N-terminally His-tagged wild-type and chimeric mutant enzymes in Escherichia coli Agrobacterium tumefaciens

Protein Variants

Protein Variants Comment Organism
additional information construction of chimeric enzymes between Thermotoga maritima and Agrobacterium tumefaciens beta-glucosidases, three chimeric genes are constructed by shuffling at three selected regions, only two chimeric enzymes are active and show altered properties, e.g. decreased thermal stability, pH optima, and kinetics, overview Agrobacterium tumefaciens
additional information construction of chimeric enzymes between Thermotoga maritima and Agrobacterium tumefaciens beta-glucosidases, three chimeric genes are constructed by shuffling at three selected regions, only two chimeric enzymes are active and show altered properties, e.g. decreased thermal stability, pH optima, and kinetics, overview Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
4-nitrophenyl-beta-D-glucopyranoside pH 6.5, 30°C, wild-type enzyme Thermotoga maritima
0.005
-
4-nitrophenyl-beta-D-xylopyranoside pH 6.5, 30°C, wild-type enzyme Agrobacterium tumefaciens
0.012
-
4-nitrophenyl-beta-D-glucopyranoside pH 6.5, 30°C, wild-type enzyme Agrobacterium tumefaciens
0.08
-
4-nitrophenyl-beta-D-fucopyranoside pH 6.5, 30°C, wild-type enzyme Agrobacterium tumefaciens
0.24
-
4-nitrophenyl-alpha-L-arabinopyranoside pH 6.5, 30°C, wild-type enzyme Agrobacterium tumefaciens
2.64
-
4-nitrophenyl-beta-D-xylopyranoside pH 6.5, 30°C, wild-type enzyme Thermotoga maritima
18.9
-
4-nitrophenyl-alpha-L-arabinopyranoside pH 6.5, 30°C, wild-type enzyme Thermotoga maritima
42.7
-
4-nitrophenyl-beta-D-fucopyranoside pH 6.5, 30°C, wild-type enzyme Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
gene cbg1
-
Agrobacterium tumefaciens B3/73
-
gene cbg1
-
Thermotoga maritima
-
gene bglb
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
gene bglb
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged wild-type and chimeric mutant enzymes from Escherichia coli by nickel affinity chromatography Agrobacterium tumefaciens
recombinant N-terminally His-tagged wild-type and chimeric mutant enzymes from Escherichia coli by nickel affinity chromatography Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
substrate specificity of wild-type and chimeric mutant enzymes, overview Agrobacterium tumefaciens
additional information
-
substrate specificity of wild-type and chimeric mutant enzymes, overview Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-fucopyranoside + H2O
-
Agrobacterium tumefaciens 4-nitrophenol + beta-D-fucose
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
Thermotoga maritima 4-nitrophenol + beta-D-fucose
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
Agrobacterium tumefaciens B3/73 4-nitrophenol + beta-D-fucose
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 4-nitrophenol + beta-D-fucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Agrobacterium tumefaciens 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermotoga maritima 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Agrobacterium tumefaciens B3/73 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Agrobacterium tumefaciens 4-nitrophenol + beta-D-xylose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Thermotoga maritima 4-nitrophenol + beta-D-xylose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Agrobacterium tumefaciens B3/73 4-nitrophenol + beta-D-xylose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 4-nitrophenol + beta-D-xylose
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
-
Agrobacterium tumefaciens 4-nitrophenol + alpha-L-arabinose
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
-
Thermotoga maritima 4-nitrophenol + alpha-L-arabinose
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
-
Agrobacterium tumefaciens B3/73 4-nitrophenol + alpha-L-arabinose
-
?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 4-nitrophenol + alpha-L-arabinose
-
?
additional information substrate specificity of wild-type and chimeric mutant enzymes, overview Agrobacterium tumefaciens ?
-
?
additional information substrate specificity of wild-type and chimeric mutant enzymes, overview Thermotoga maritima ?
-
?
additional information substrate specificity of wild-type and chimeric mutant enzymes, overview Agrobacterium tumefaciens B3/73 ?
-
?
additional information substrate specificity of wild-type and chimeric mutant enzymes, overview Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the glycosyl hydrolase family 3 Agrobacterium tumefaciens
More the enzyme belongs to the glycosyl hydrolase family 3 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Agrobacterium tumefaciens
85
-
-
Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.4
-
4-nitrophenyl-beta-D-glucopyranoside pH 6.5, 30°C, wild-type enzyme Thermotoga maritima
9
-
4-nitrophenyl-alpha-L-arabinopyranoside pH 6.5, 30°C, wild-type enzyme Thermotoga maritima
18.4
-
4-nitrophenyl-beta-D-xylopyranoside pH 6.5, 30°C, wild-type enzyme Thermotoga maritima
22.1
-
4-nitrophenyl-beta-D-fucopyranoside pH 6.5, 30°C, wild-type enzyme Agrobacterium tumefaciens
27.7
-
4-nitrophenyl-beta-D-fucopyranoside pH 6.5, 30°C, wild-type enzyme Thermotoga maritima
28.9
-
4-nitrophenyl-beta-D-xylopyranoside pH 6.5, 30°C, wild-type enzyme Agrobacterium tumefaciens
95.4
-
4-nitrophenyl-beta-D-glucopyranoside pH 6.5, 30°C, wild-type enzyme Agrobacterium tumefaciens
119
-
4-nitrophenyl-alpha-L-arabinopyranoside pH 6.5, 30°C, wild-type enzyme Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2 3.5
-
Thermotoga maritima
6.5 8
-
Agrobacterium tumefaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 11
-
Thermotoga maritima