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Literature summary for 3.2.1.21 extracted from
- Purification method improvement and characterization of a novel ginsenoside-hydrolyzing beta-glucosidase from Paecilomyces Bainier sp. 229 (2008), Biosci. Biotechnol. Biochem., 72, 352-359.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.072 | - |
ginsenoside Rb1 | pH 3.5, 45°C | Paecilomyces sp. (in: Eurotiomycetes) |  |
| 0.111 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 3.5, 45°C | Paecilomyces sp. (in: Eurotiomycetes) | |
| 0.805 | - |
ginsenoside Rd | pH 3.5, 45°C | Paecilomyces sp. (in: Eurotiomycetes) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 102000 | - |
3 * 102000, SDS-PAGE | Paecilomyces sp. (in: Eurotiomycetes) |
| 305000 | - |
gel filtration | Paecilomyces sp. (in: Eurotiomycetes) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paecilomyces sp. (in: Eurotiomycetes) | - |
- |
- |
| Paecilomyces sp. (in: Eurotiomycetes) 229 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 144fold by ammonium sulfate fractionation, anion exchange chromatography, and affinity chromatography on microcrystalline cellulose, to homogeneity, method optimization, overview | Paecilomyces sp. (in: Eurotiomycetes) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 56.7 | - |
purified enzyme, substrate ginsenoside Rb1 | Paecilomyces sp. (in: Eurotiomycetes) |
| 129.4 | - |
purified enzyme, substrate ginsenoside Rd | Paecilomyces sp. (in: Eurotiomycetes) |
| 170.5 | - |
purified enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside | Paecilomyces sp. (in: Eurotiomycetes) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Paecilomyces sp. (in: Eurotiomycetes) | 4-nitrophenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Paecilomyces sp. (in: Eurotiomycetes) 229 | 4-nitrophenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Paecilomyces sp. (in: Eurotiomycetes) 220 Bainier | 4-nitrophenol + beta-D-glucose | - |
? | |
| cellobiose + H2O | low activity | Paecilomyces sp. (in: Eurotiomycetes) | beta-D-glucose + alpha-D-glucose | - |
? | |
| cellobiose + H2O | low activity | Paecilomyces sp. (in: Eurotiomycetes) 229 | beta-D-glucose + alpha-D-glucose | - |
? | |
| cellobiose + H2O | low activity | Paecilomyces sp. (in: Eurotiomycetes) 220 Bainier | beta-D-glucose + alpha-D-glucose | - |
? | |
| gentiobiose + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) | 2 beta-D-glucose | - |
? | |
| gentiobiose + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) 229 | 2 beta-D-glucose | - |
? | |
| gentiobiose + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) 220 Bainier | 2 beta-D-glucose | - |
? | |
| ginsenoside Rb1 + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) | ? | - |
? | |
| ginsenoside Rd + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) | ? | - |
? | |
| additional information | maltose is a poor substrate, no activity with ginsenoside Rg3, Rb2, and Rb3, and with 4-nitrophenyl-alpha-D-glucopyranoside and 4-nitrophenyl-beta-D-xylopyranoside | Paecilomyces sp. (in: Eurotiomycetes) | ? | - |
? | |
| additional information | maltose is a poor substrate, no activity with ginsenoside Rg3, Rb2, and Rb3, and with 4-nitrophenyl-alpha-D-glucopyranoside and 4-nitrophenyl-beta-D-xylopyranoside | Paecilomyces sp. (in: Eurotiomycetes) 229 | ? | - |
? | |
| additional information | maltose is a poor substrate, no activity with ginsenoside Rg3, Rb2, and Rb3, and with 4-nitrophenyl-alpha-D-glucopyranoside and 4-nitrophenyl-beta-D-xylopyranoside | Paecilomyces sp. (in: Eurotiomycetes) 220 Bainier | ? | - |
? | |
| sophorose + H2O | low activity | Paecilomyces sp. (in: Eurotiomycetes) | 2 beta-D-glucose | - |
? | |
| sophorose + H2O | low activity | Paecilomyces sp. (in: Eurotiomycetes) 229 | 2 beta-D-glucose | - |
? | |
| sophorose + H2O | low activity | Paecilomyces sp. (in: Eurotiomycetes) 220 Bainier | 2 beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 102000, SDS-PAGE | Paecilomyces sp. (in: Eurotiomycetes) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Paecilomyces sp. (in: Eurotiomycetes) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
purified enzyme, 4 h, pH 3.5, stable up to, rapid decrease in activity above | Paecilomyces sp. (in: Eurotiomycetes) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Paecilomyces sp. (in: Eurotiomycetes) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 4 | rapid decrease in activity below pH 3.0 and above pH 4.0 | Paecilomyces sp. (in: Eurotiomycetes) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | purified enzyme, 4 h, 45°C, stable | Paecilomyces sp. (in: Eurotiomycetes) |
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