Application | Comment | Organism |
---|---|---|
industry | application in simultaneous saccharification and fermentation, evaluation of the suitability of plant glycosyl hydrolases in lignocellulose conversion | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Zea mays | |
cellobiose | competitive versus substrate salicin | Zea mays | |
Cu2+ | - |
Zea mays | |
D-glucose | competitive versus substrate 4-nitrophenyl-beta-D-glucopyranoside | Zea mays | |
Hg2+ | - |
Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 4.5, 37°C | Zea mays | |
4.6 | - |
cellobiose | pH 4.5, 37°C | Zea mays | |
9.3 | - |
Salicin | pH 4.5, 37°C | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates | Zea mays | |
Ca2+ | activates | Zea mays | |
K+ | activates | Zea mays | |
Mg2+ | activates | Zea mays | |
additional information | no effects by EDTA and NaCl | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62400 | - |
native PAGE | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 45fold to homogeneity by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration | Zea mays |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.5 | - |
purified enzyme | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Zea mays | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Zea mays | 2 beta-D-glucose | - |
? | |
salicin + H2O | - |
Zea mays | beta-D-glucose + 2-(hydroxymethyl)phenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62400, SDS-PAGE | Zea mays |
More | capillary LCESI/MS peptide sequence determination and protein identification | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
salicinase | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Zea mays |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | - |
Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | stable within this range, rapid decrease in activity below 30°C and above 40°C | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.6 | 6 | - |
Zea mays |