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Literature summary for 3.2.1.21 extracted from

  • Han, Y.; Chen, H.
    Characterization of beta-glucosidase from corn stover and its application in simultaneous saccharification and fermentation (2008), Biores. Technol., 99, 6081-6087.
    View publication on PubMed

Application

Application Comment Organism
industry application in simultaneous saccharification and fermentation, evaluation of the suitability of plant glycosyl hydrolases in lignocellulose conversion Zea mays

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Zea mays
cellobiose competitive versus substrate salicin Zea mays
Cu2+
-
Zea mays
D-glucose competitive versus substrate 4-nitrophenyl-beta-D-glucopyranoside Zea mays
Hg2+
-
Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
4-nitrophenyl-beta-D-glucopyranoside pH 4.5, 37°C Zea mays
4.6
-
cellobiose pH 4.5, 37°C Zea mays
9.3
-
Salicin pH 4.5, 37°C Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
2-mercaptoethanol activates Zea mays
Ca2+ activates Zea mays
K+ activates Zea mays
Mg2+ activates Zea mays
additional information no effects by EDTA and NaCl Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62400
-
native PAGE Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 45fold to homogeneity by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration Zea mays

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.5
-
purified enzyme Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Zea mays 4-nitrophenol + beta-D-glucose
-
?
cellobiose + H2O
-
Zea mays 2 beta-D-glucose
-
?
salicin + H2O
-
Zea mays beta-D-glucose + 2-(hydroxymethyl)phenol
-
?

Subunits

Subunits Comment Organism
monomer 1 * 62400, SDS-PAGE Zea mays
More capillary LC–ESI/MS peptide sequence determination and protein identification Zea mays

Synonyms

Synonyms Comment Organism
salicinase
-
Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Zea mays

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60
-
Zea mays

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 stable within this range, rapid decrease in activity below 30°C and above 40°C Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
Zea mays

pH Range

pH Minimum pH Maximum Comment Organism
3.6 6
-
Zea mays