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Literature summary for 3.2.1.21 extracted from

  • Seidle, H.F.; Allison, S.J.; George, E.; Huber, R.E.
    Trp-49 of the family 3 beta-glucosidase from Aspergillus niger is important for its transglucosidic activity: creation of novel beta-glucosidases with low transglucosidic efficiencies (2006), Arch. Biochem. Biophys., 455, 110-118.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
methanol-inducible expression of wild-type and mutant enzymes in Pichia pastoris strain X-33 Aspergillus niger

Protein Variants

Protein Variants Comment Organism
W49A site-directed mutagenesis, the mutation reduces the transglucosylation activity of the enzyme due to a large decrease in binding capacity for Glc at site +1 and an increase in binding ability at site-1, while the hydrolytic activity is only slightly decreased compared to the wild-type enzyme, the mutant shows altered kinetics, overview Aspergillus niger
W49D site-directed mutagenesis, the mutation reduces the transglucosylation activity of the enzyme due to a large decrease in binding capacity for Glc at site +1 and an increase in binding ability at site-1, while the hydrolytic activity is only slightly decreased compared to the wild-type enzyme, the mutant shows altered kinetics, overview Aspergillus niger
W49G site-directed mutagenesis, the mutation reduces the transglucosylation activity of the enzyme due to a large decrease in binding capacity for Glc at site +1 and an increase in binding ability at site-1, while the hydrolytic activity is only slightly decreased compared to the wild-type enzyme, the mutant shows altered kinetics, overview Aspergillus niger
W49N site-directed mutagenesis, the mutation reduces the transglucosylation activity of the enzyme due to a large decrease in binding capacity for Glc at site +1 and an increase in binding ability at site-1, while the hydrolytic activity is only slightly decreased compared to the wild-type enzyme, the mutant shows altered kinetics, overview Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive inhibition of wild-type and mutant enzymes Aspergillus niger
D-gluconolactone competitive inhibition of wild-type enzyme and mutant W49A Aspergillus niger
D-glucose competitive inhibition of wild-type and mutant enzymes Aspergillus niger
gentiobiose competitive inhibition of wild-type enzyme and mutant W49A Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of hydrolytic activity of mutant enzymes and of transglucosylation activities of recombinant wild-type and mutant enzymes, overview Aspergillus niger
0.27
-
 cellotetraose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
0.33
-
 cellopentaose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
0.52
-
 cellotriose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
1.5
-
 4-nitrophenyl-beta-D-glucopyranoside pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
2.2
-
 gentiobiose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
2.7
-
 cellobiose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + H2O Aspergillus niger
-
 2 beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Reaction

Reaction Comment Organism Reaction ID
celloheptaose + 6 H2O = 7 beta-D-glucose Trp49 is important for transglucosylation catalytic activity Aspergillus niger
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus niger 4-nitrophenol + beta-D-glucose
-
 ?
cellobiose + H2O
-
 Aspergillus niger 2 beta-D-glucose
-
 ?
cellopentaose + H2O
-
 Aspergillus niger 5 D-glucose
-
 ?
cellotetraose + 3 H2O
-
 Aspergillus niger 4 beta-D-glucose
-
 ?
cellotriose + H2O
-
 Aspergillus niger 3 D-glucose
-
 ?
gentiobiose + H2O
-
 Aspergillus niger 2 beta-D-glucose
-
 ?
additional information the enzyme shows transglucosylation activity involving Trp49, overview Aspergillus niger ?
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the glycosyl hydrolase family 3 Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 3.7 cellotriose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
51.7
-
 cellopentaose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
54.7
-
 cellotetraose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
70
-
 gentiobiose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
85.1
-
 cellotriose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
89
-
 4-nitrophenyl-beta-D-glucopyranoside pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
103
-
 cellobiose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 assay at Aspergillus niger

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 D-gluconolactone pH 4.5, 25°C, recombinant mutant W49A Aspergillus niger
0.4
-
 D-gluconolactone pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
1.1
-
 cellobiose pH 4.5, 25°C, recombinant mutant W49D Aspergillus niger
1.2
-
 cellobiose pH 4.5, 25°C, recombinant mutant W49N Aspergillus niger
1.3
-
 cellobiose pH 4.5, 25°C, recombinant mutant W49G Aspergillus niger
1.4
-
 cellobiose pH 4.5, 25°C, recombinant mutant W49A Aspergillus niger
1.6
-
 cellobiose pH 4.5, 25°C, recombinant mutants W49N and W49G Aspergillus niger
1.8
-
 gentiobiose pH 4.5, 25°C, recombinant mutant W49A Aspergillus niger
1.8
-
 cellobiose pH 4.5, 25°C, recombinant wild-type enzyme and mutant W49A Aspergillus niger
1.9
-
 cellobiose pH 4.5, 25°C, recombinant mutant W49D Aspergillus niger
3
-
 D-glucose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger
4.8
-
 gentiobiose pH 4.5, 25°C, recombinant wild-type enzyme Aspergillus niger