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Literature summary for 3.2.1.21 extracted from

  • Byeon, G.M.; Lee, K.S.; Gui, Z.Z.; Kim, I.; Kang, P.D.; Lee, S.M.; Sohn, H.D.; Jin, B.R.
    A digestive beta-glucosidase from the silkworm, Bombyx mori: cDNA cloning, expression and enzymatic characterization (2005), Comp. Biochem. Physiol. B, 141, 418-427.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
single gene, genomic organization, DNA and amino acid sequence determination and analysis, expression in Spodoptera frugiperda Sf9 cells using the baculovirus infection system Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori Q86D78 silkworm, class 2 enzyme
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme is N-glycosylated at residue N43 and N237, which is not essential for activity Bombyx mori

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 1112.2fold from Spodoptera frugiperda Sf9 cells by ammonium sulfate fractionation, gel filtration and anion exchange chromatography, cleavage of the signal peptide Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
midgut larval, specific expression only during feeding period Bombyx mori
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
665
-
 purified recombinant enzyme, substrate cellobiose Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + H2O recombinant enzyme Bombyx mori 2 D-glucose
-
 ?
lactose + H2O recombinant enzyme Bombyx mori D-glucose + D-galactose
-
 ?
additional information no activity of the recombinant enzyme with salicin Bombyx mori ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 recombinant enzyme Bombyx mori

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 48
-
 Bombyx mori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable for at least 10 min, recombinant enzyme Bombyx mori
55
-
 inactivation, recombinant enzyme Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 recombinant enzyme Bombyx mori

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
 Bombyx mori

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 recombinant enzyme, stable Bombyx mori

pI Value

Organism Comment pI Value Maximum pI Value
Bombyx mori amino acid sequence calculation
-
 4.8