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Literature summary for 3.2.1.21 extracted from

  • Harada, K.M.; Tanaka, K.; Fukuda, Y.; Hashimoto, W.; Murata, K.
    Degradation of rice bran hemicellulose by Paenibacillus sp. strain HC1: gene cloning, characterization and function of beta-D-glucosidase as an enzyme involved in degradation (2005), Arch. Microbiol., 184, 215-224.
    View publication on PubMed
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is induced by growth on rice bran hemicellulose, and slightly by pectin, xylan, and glucose, no effect by EDTA, L-fucose, G-glucuronic acid, D-mannose, L-rhamnose, and D-xylose Paenibacillus sp.

Application

Application Comment Organism
nutrition the enzyme is useful in rice polishing by degradation and assimilation of rice bran hemicellulose of the outer grain skin Paenibacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene bglA, DNA and amino acid sequence determination and analysis, cDNA library construction, expression in Escherichi coli Paenibacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 12% inhibition at 1 mM Paenibacillus sp.
Cu2+ 79% inhibition at 1 mM Paenibacillus sp.
D-galactose 46% inhibition at 1.5 mM Paenibacillus sp.
D-glucose 76% inhibition at 1.5 mM Paenibacillus sp.
D-glucuronic acid 12% inhibition at 1.5 mM Paenibacillus sp.
D-mannose 10% inhibition at 1.5 mM Paenibacillus sp.
Fe2+ 63% inhibition at 1 mM Paenibacillus sp.
Hg2+ 99% inhibition at 1 mM Paenibacillus sp.
Mn2+ 40% inhibition at 1 mM Paenibacillus sp.
additional information no effect by EDTA, DTT, 2-mercaptoethanol, iodoacetc acid, glutathione, L-fucose, L-rhamnose, and D-xylose Paenibacillus sp.
NEM 26% inhibition at 1 mM Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.1
-
 4-nitrophenyl beta-D-glucopyranoside pH 7.0, 30°C, recombinant enzyme Paenibacillus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Paenibacillus sp. 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 14% activation at 1 mM Paenibacillus sp.
additional information the enzyme is not affected by Mg2+ Paenibacillus sp.
Zn2+ 11% activation at 1 mM Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 recombinant enzyme, gel filtration Paenibacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rice bran hemicellulose + H2O Paenibacillus sp. the enzyme is involved in degradation ?
-
 ?
rice bran hemicellulose + H2O Paenibacillus sp. HC1 the enzyme is involved in degradation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. Q2WGB4 isolated from soil, gene bglA
-
Paenibacillus sp. HC1 Q2WGB4 isolated from soil, gene bglA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant BglA 52.9fold from Escherichia coli by ammonium sulfate fractionation, ion exchange and adsorption chromatography, and gel filtration Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
39.4
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, enzyme expression induced by growth on rice bran hemicellulose Paenibacillus sp.
74.6
-
 purified enzyme Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Paenibacillus sp. 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Paenibacillus sp. 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Paenibacillus sp. HC1 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Paenibacillus sp. 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Paenibacillus sp. HC1 4-nitrophenol + beta-D-glucose
-
 ?
additional information substrate specificity, overview Paenibacillus sp. ?
-
 ?
additional information substrate specificity, overview Paenibacillus sp. HC1 ?
-
 ?
rice bran hemicellulose + H2O degradation Paenibacillus sp. ?
-
 ?
rice bran hemicellulose + H2O the enzyme is involved in degradation Paenibacillus sp. ?
-
 ?
rice bran hemicellulose + H2O degradation Paenibacillus sp. HC1 ?
-
 ?
rice bran hemicellulose + H2O the enzyme is involved in degradation Paenibacillus sp. HC1 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, recombinant enzyme, SDS-PAGE Paenibacillus sp.

Synonyms

Synonyms Comment Organism
BglA
-
 Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 10 min, loss of 50% activity Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Paenibacillus sp.