Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant BglC in Escherichia coli | Thermobifida fusca |
Protein Variants | Comment | Organism |
---|---|---|
A433V | random mutagenesis, identification of the thermostable mutant, kinetics in comparison to the wild-type enzyme | Thermobifida fusca |
L444F | random mutagenesis, identification of the thermostable mutant, kinetics in comparison to the wild-type enzyme | Thermobifida fusca |
N178I | random mutagenesis, identification of the thermostable mutant, substrate-saturation study, binding of a second substrate molecule increases the turnover with D-cellobiose | Thermobifida fusca |
N317Y | random mutagenesis, identification of the thermostable mutant, kinetics in comparison to the wild-type enzyme | Thermobifida fusca |
N317Y/L444F | random mutagenesis, identification of the thermostable mutant, substrate-saturation study, binding of a second substrate molecule increases the turnover with D-cellobiose | Thermobifida fusca |
N317Y/L444F/A422V | random mutagenesis, identification of the thermostable mutant, substrate-saturation study, binding of a second substrate molecule increases the turnover with D-cellobiose | Thermobifida fusca |
N439S | random mutagenesis, identification of the thermostable mutant, kinetics in comparison to the wild-type enzyme | Thermobifida fusca |
S319C | random mutagenesis, identification of the thermostable mutant, the mutant shows highly increased thermotolerance and activity with D-cellobiose compared to the wild-type enzyme | Thermobifida fusca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamics and kinetics of wild-type and mutant enzymes, isothermal titration microcalorimetry | Thermobifida fusca | |
0.19 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant A433V | Thermobifida fusca | |
0.21 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant N317Y | Thermobifida fusca | |
0.22 | - |
D-cellobiose | pH 7.2, 25°C, recombinant wild-type enzyme | Thermobifida fusca | |
0.23 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant N439S | Thermobifida fusca | |
0.31 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant L444F | Thermobifida fusca | |
0.4 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant S319C | Thermobifida fusca | |
0.41 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant N317Y/L444F | Thermobifida fusca | |
0.63 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant N178I | Thermobifida fusca | |
0.66 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant N317Y/N439S | Thermobifida fusca | |
1.01 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant N317Y/L444F/A433V | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
gene bglC | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
celloheptaose + 6 H2O = 7 beta-D-glucose | reaction and kinetic mechanisms | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-cellobiose + H2O | - |
Thermobifida fusca | beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BglC | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Thermobifida fusca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermostability of wild-type and mutant enzymes, overview | Thermobifida fusca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant L444F | Thermobifida fusca | |
4.3 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant N317Y/N439S | Thermobifida fusca | |
12.3 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant A433V | Thermobifida fusca | |
14 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant N317Y | Thermobifida fusca | |
16.3 | - |
D-cellobiose | pH 7.2, 25°C, recombinant wild-type enzyme | Thermobifida fusca | |
16.8 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant N317Y/L444F | Thermobifida fusca | |
19.2 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant N317Y/L444F/A433V | Thermobifida fusca | |
21 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant S319C | Thermobifida fusca | |
22.3 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant N178I | Thermobifida fusca | |
29.5 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant N439S | Thermobifida fusca | |
111.3 | - |
D-cellobiose | pH 7.2, 25°C, recombinant mutant S319C | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Thermobifida fusca |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate inhibition kinetics of wild-type and mutant enzymes, overview | Thermobifida fusca |