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Literature summary for 3.2.1.21 extracted from

  • Jeoh, T.; Baker, J.O.; Ali, M.K.; Himmel, M.E.; Adney, W.S.
    beta-D-Glucosidase reaction kinetics from isothermal titration microcalorimetry (2005), Anal. Biochem., 347, 244-253.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant BglC in Escherichia coli Thermobifida fusca

Protein Variants

Protein Variants Comment Organism
A433V random mutagenesis, identification of the thermostable mutant, kinetics in comparison to the wild-type enzyme Thermobifida fusca
L444F random mutagenesis, identification of the thermostable mutant, kinetics in comparison to the wild-type enzyme Thermobifida fusca
N178I random mutagenesis, identification of the thermostable mutant, substrate-saturation study, binding of a second substrate molecule increases the turnover with D-cellobiose Thermobifida fusca
N317Y random mutagenesis, identification of the thermostable mutant, kinetics in comparison to the wild-type enzyme Thermobifida fusca
N317Y/L444F random mutagenesis, identification of the thermostable mutant, substrate-saturation study, binding of a second substrate molecule increases the turnover with D-cellobiose Thermobifida fusca
N317Y/L444F/A422V random mutagenesis, identification of the thermostable mutant, substrate-saturation study, binding of a second substrate molecule increases the turnover with D-cellobiose Thermobifida fusca
N439S random mutagenesis, identification of the thermostable mutant, kinetics in comparison to the wild-type enzyme Thermobifida fusca
S319C random mutagenesis, identification of the thermostable mutant, the mutant shows highly increased thermotolerance and activity with D-cellobiose compared to the wild-type enzyme Thermobifida fusca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information thermodynamics and kinetics of wild-type and mutant enzymes, isothermal titration microcalorimetry Thermobifida fusca
0.19
-
D-cellobiose pH 7.2, 25°C, recombinant mutant A433V Thermobifida fusca
0.21
-
D-cellobiose pH 7.2, 25°C, recombinant mutant N317Y Thermobifida fusca
0.22
-
D-cellobiose pH 7.2, 25°C, recombinant wild-type enzyme Thermobifida fusca
0.23
-
D-cellobiose pH 7.2, 25°C, recombinant mutant N439S Thermobifida fusca
0.31
-
D-cellobiose pH 7.2, 25°C, recombinant mutant L444F Thermobifida fusca
0.4
-
D-cellobiose pH 7.2, 25°C, recombinant mutant S319C Thermobifida fusca
0.41
-
D-cellobiose pH 7.2, 25°C, recombinant mutant N317Y/L444F Thermobifida fusca
0.63
-
D-cellobiose pH 7.2, 25°C, recombinant mutant N178I Thermobifida fusca
0.66
-
D-cellobiose pH 7.2, 25°C, recombinant mutant N317Y/N439S Thermobifida fusca
1.01
-
D-cellobiose pH 7.2, 25°C, recombinant mutant N317Y/L444F/A433V Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
gene bglC
-

Reaction

Reaction Comment Organism Reaction ID
celloheptaose + 6 H2O = 7 beta-D-glucose reaction and kinetic mechanisms Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-cellobiose + H2O
-
Thermobifida fusca beta-D-glucose
-
?

Synonyms

Synonyms Comment Organism
BglC
-
Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermostability of wild-type and mutant enzymes, overview Thermobifida fusca

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6
-
D-cellobiose pH 7.2, 25°C, recombinant mutant L444F Thermobifida fusca
4.3
-
D-cellobiose pH 7.2, 25°C, recombinant mutant N317Y/N439S Thermobifida fusca
12.3
-
D-cellobiose pH 7.2, 25°C, recombinant mutant A433V Thermobifida fusca
14
-
D-cellobiose pH 7.2, 25°C, recombinant mutant N317Y Thermobifida fusca
16.3
-
D-cellobiose pH 7.2, 25°C, recombinant wild-type enzyme Thermobifida fusca
16.8
-
D-cellobiose pH 7.2, 25°C, recombinant mutant N317Y/L444F Thermobifida fusca
19.2
-
D-cellobiose pH 7.2, 25°C, recombinant mutant N317Y/L444F/A433V Thermobifida fusca
21
-
D-cellobiose pH 7.2, 25°C, recombinant mutant S319C Thermobifida fusca
22.3
-
D-cellobiose pH 7.2, 25°C, recombinant mutant N178I Thermobifida fusca
29.5
-
D-cellobiose pH 7.2, 25°C, recombinant mutant N439S Thermobifida fusca
111.3
-
D-cellobiose pH 7.2, 25°C, recombinant mutant S319C Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Thermobifida fusca

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information substrate inhibition kinetics of wild-type and mutant enzymes, overview Thermobifida fusca