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Literature summary for 3.2.1.21 extracted from

  • Odoux, E.; Chauwin, A.; Brillouet, J.M.
    Purification and characterization of vanilla bean (Vanilla planifolia Andrews) beta-D-glucosidase (2003), J. Agric. Food Chem., 51, 3168-3173.
    View publication on PubMed
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 500 mM, 2fold activation, inhibition above Vanilla planifolia

Inhibitors

Inhibitors Comment Organism Structure
1,4-dithiothreitol 1.5-2 M, complete inhibition Vanilla planifolia
1-deoxynojirimycin
-
 Vanilla planifolia
2-mercaptoethanol 2 M, 95% inhibition Vanilla planifolia
Ag+ 1 mM, 81% inhibition Vanilla planifolia
Co2+ 1 mM, 53% inhibition Vanilla planifolia
Cu2+ 1 mM, 96% inhibition Vanilla planifolia
glucono-delta-lactone
-
 Vanilla planifolia
Hg2+ 0.06 mM, 97% inhibition Vanilla planifolia
Mn2+ 1 mM, 66% inhibition Vanilla planifolia
additional information not inhibited by D-glucose up to 2 M Vanilla planifolia
Ni2+ 1 mM, 70% inhibition Vanilla planifolia
Zn2+ 1 mM, 93% inhibition Vanilla planifolia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 p-nitrophenyl-beta-D-glucopyranoside 40°C, pH 7.0 Vanilla planifolia
20
-
 glucovanillin 40°C, pH 7.0 Vanilla planifolia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 50000, SDS-PAGE Vanilla planifolia
201000
-
 gel filtration Vanilla planifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glucovanillin + H2O Vanilla planifolia
-
 vanillin + beta-D-glucose
-
 ?
glucovanillin + H2O Vanilla planifolia Andrews
-
 vanillin + beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Vanilla planifolia
-
 Andrews
-
Vanilla planifolia Andrews
-
 Andrews
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose, Phenyl-Sepharose, Sephacryl S-300 HR Vanilla planifolia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
177.6
-
-
 Vanilla planifolia

Storage Stability

Storage Stability Organism
4°C, 20 mM Tris-HCl, pH 8.0, at least 6 months, no loss of activity Vanilla planifolia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
esculin + H2O 92% of activity with p-nitrophenyl-beta-D-glucopyranoside Vanilla planifolia beta-D-glucose + esculetin
-
 ?
esculin + H2O 92% of activity with p-nitrophenyl-beta-D-glucopyranoside Vanilla planifolia Andrews beta-D-glucose + esculetin
-
 ?
glucovanillin + H2O
-
 Vanilla planifolia vanillin + beta-D-glucose
-
 ?
glucovanillin + H2O 22% of activity with p-nitrophenyl-beta-D-glucopyranoside Vanilla planifolia vanillin + beta-D-glucose
-
 ?
glucovanillin + H2O
-
 Vanilla planifolia Andrews vanillin + beta-D-glucose
-
 ?
glucovanillin + H2O 22% of activity with p-nitrophenyl-beta-D-glucopyranoside Vanilla planifolia Andrews vanillin + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-fucopyranoside + H2O 215% of activity with p-nitrophenyl-beta-D-glucopyranoside Vanilla planifolia p-nitrophenol + beta-D-fucose
-
 ?
p-nitrophenyl-beta-D-fucopyranoside + H2O 215% of activity with p-nitrophenyl-beta-D-glucopyranoside Vanilla planifolia Andrews p-nitrophenol + beta-D-fucose
-
 ?
p-nitrophenyl-beta-D-galactopyranoside + H2O 61% of activity with p-nitrophenyl-beta-D-glucopyranoside Vanilla planifolia p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Vanilla planifolia p-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-xylopyranoside + H2O 5% of activity with p-nitrophenyl-beta-D-glucopyranoside Vanilla planifolia p-nitrophenol + beta-D-xylose
-
 ?
prunasin + H2O 300% of activity with p-nitrophenyl-beta-D-glucopyranoside Vanilla planifolia D-mandelonitrile + beta-D-glucose
-
 ?
prunasin + H2O 300% of activity with p-nitrophenyl-beta-D-glucopyranoside Vanilla planifolia Andrews D-mandelonitrile + beta-D-glucose
-
 ?
salicin + H2O 4% of activity with p-nitrophenyl-beta-D-glucopyranoside Vanilla planifolia beta-D-glucose + salicylic alcohol
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, SDS-PAGE Vanilla planifolia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Vanilla planifolia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 approx. 25% of maximal activity at 20°C, approx. 15% of maximal activity at 70°C Vanilla planifolia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 no loss of activity after 30 min Vanilla planifolia
50
-
 approx. 40% loss of activity after 30 min Vanilla planifolia
60
-
 approx. 80% loss of activity after 30 min Vanilla planifolia
70
-
 complete loss of activity after 30 min Vanilla planifolia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 very sharp optimum Vanilla planifolia

pH Range

pH Minimum pH Maximum Comment Organism
5 9 approx. 5% of maximal activity at pH 5.5 and pH 9.0, respectively Vanilla planifolia

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 12 complete loss of activity below pH 6.0 after 4 h at 20°C, 37% loss of activity after 4 h at pH 12.0 and 20°C Vanilla planifolia

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.15
-
 1-deoxynojirimycin 40°C, pH 7.0 Vanilla planifolia
0.67
-
 glucono-delta-lactone 40°C, pH 7.0 Vanilla planifolia